| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Poly(A) Polymerase, E. coli (E-PAP) catalyzes the template-independent addition of adenosine residues to the 3’ ends of RNA, efficiently creating a poly(A) tail. Unlike yeast-derived enzymes, E-PAP works in a sequence-independent manner, meaning it does not require specific recognition sequences to function. This allows for rapid polyadenylation at virtually any unprotected 3’ RNA terminus, regardless of secondary structure. E-PAP is ideal for applications that require poly(A) tail addition to a wide range of RNA molecules, including RNA sequencing and cDNA synthesis. Its versatility and efficiency make it a valuable tool for researchers working with RNA in various experimental setups.
| Product Component | Quantity |
|---|---|
| Poly(A) Polymerase, E. coli | 25 µl |
| 10X Poly(A) Polymerase, E. coli Reaction Buffer | 1.0 ml |
| ATP (10 mM) | 50 µl |
| Specification | Value |
|---|---|
| Enzyme Type | Poly(A) Polymerase |
| Format | Enzyme Only |
| Storage Conditions | Store all components at -20°C. |
Poly(A) Polymerase adds adenosine residues in a template-independent manner to the 3′-OH end of single-stranded RNA, preferring single-stranded RNA substrates. It requires ATP and Mg²⁺ for activity and is used for 3′-end labeling, poly(A) tailing for in vitro translation, and RNA detection assays.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Gomaa A; Peng D; Chen Z et al.. Epigenetic regulation of AURKA by miR-4715-3p in upper gastrointestinal cancers. Scientific reports. 2019;9:16970. DOI
- Shao L; Chen Z; Peng D et al.. Methylation of the HOXA10 Promoter Directs miR-196b-5p-Dependent Cell Proliferation and Invasion of Gastric Cancer Cells. Molecular cancer research : MCR. 2018;16:696-706. DOI
