| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Sample type(s) | Serum | Plasma |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Porcine Taenia Solium IgG ELISA Kit quantifies porcine IgG antibodies against Taenia Solium in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.
Values are expressed relative to the antibody standard supplied with the kit.
Specifications
| Assay Type | Quantitative |
|---|---|
| Detection Method | Colorimetric (OD 450 nm) |
| Sample Type | Serum, Plasma |
| Sensitivity | 0.25 ng/mL |
| Detection Range | 1.56 - 100 ng/mL |
| Recovery | 80-120% |
| Kit Size | 96 T |
| Shipping | 2-8 °C |
Kit Components
| Component | Quantity |
|---|---|
| ELISA Microtiter plate | 96 wells |
| Dilution plate | 96 wells |
| HRP Conjugate | 10 mL |
| Sample Diluent | 6 mL |
| 20×Concentrated Wash Buffer | 25 mL |
| Substrate Reagent A | 6 mL |
| Substrate Reagent B | 6 mL |
| Stop Solution | 6 mL |
| Positive Control | 0.5 mL |
| Negative Control | 0.5mL |
| Plate Sealer | 3 pieces |
| Sealed Bag | 1 piece |
| Manual | 1 copy |
Assay Procedure
Bring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.
- Remove the required number of strips from the foil pouch after equilibration to room temperature for 20 minutes. Return unused strips to the resealable pouch and store at 4 °C.
- Set negative control wells, positive control wells, and sample wells. Add 50 µL of negative control and positive control to their respective wells.
- For test sample wells, first add 10 µL of the test sample, then add 40 µL of sample diluent.
- Then add 100 µL of HRP labelled detection antibody to each well (including control and sample wells). Cover the plate with the sealing film and incubate at 37 °C for 60 minutes in a water bath or incubator.
- Discard the liquid, tap dry on absorbent paper. Fill each well with wash buffer, let stand for 1 minute, discard the wash buffer, and tap dry on absorbent paper. Repeat the wash 5 times (an automatic plate washer may also be used).
- Add 50 µL of Substrate A and 50 µL of Substrate B to each well. Incubate at 37 °C in the dark for 15 minutes.
- Add 50 µL of Stop Solution to each well. Measure the OD of each well at 450 nm within 15 minutes.
Required but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm / 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
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