| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
Peroxisome proliferator-activated receptor alpha (PPAR-alpha) is a ligand-activated nuclear receptor and a central regulator of lipid metabolism. Highly expressed in the liver, it is activated by fatty acids and their derivatives and heterodimerizes with the retinoid X receptor to bind PPAR response elements (PPRE) in target gene promoters. PPAR-alpha drives the transcription of genes controlling fatty acid uptake, mitochondrial and peroxisomal beta-oxidation, and ketogenesis, particularly during fasting. It is the molecular target of fibrate drugs used to lower circulating lipids. Because of its role in energy homeostasis and lipid handling, PPAR-alpha is an important focus of research into metabolic disorders, fatty liver disease, and diabetes.
Product Description & Applications
The PPAR-alpha Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PPAR-alpha activity in mammalian cells. The construct contains tandem repeats of the PPRE element from the Acox1 promoter placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. The design favors activation by PPAR-alpha and RXR-alpha heterodimers and responds more strongly to PPAR-alpha than to PPAR-gamma. A constitutive drug selection marker (Puromycin or Blasticidin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying PPAR signaling in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the PPARa transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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