| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
Peroxisome proliferator-activated receptor gamma (PPAR-gamma) is a ligand-activated nuclear receptor and a master regulator of adipocyte differentiation and lipid storage. Activated by fatty acid derivatives and synthetic agonists, PPAR-gamma heterodimerizes with the retinoid X receptor and binds PPAR response elements (PPRE) to control genes governing adipogenesis, glucose homeostasis, and insulin sensitivity. It is also expressed in macrophages and other cell types, where it modulates inflammatory responses. PPAR-gamma is the molecular target of thiazolidinedione drugs used to improve insulin sensitivity, and its activity is central to studies of obesity, type 2 diabetes, and metabolic disease, making it a key focus of metabolism and endocrinology research.
Product Description & Applications
The PPAR-gamma Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PPAR-gamma activity in transduced cells. The construct places a reporter gene (firefly luciferase, GFP, RFP, or Renilla luciferase) under the control of tandem PPAR response elements optimized for preferential activation by PPAR-gamma over the PPAR-alpha and PPAR-delta isoforms, coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying PPAR-gamma activation in primary and difficult-to-transfect cells for metabolism and endocrinology research.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
