PPP2R2B Antibody, FITC conjugated

Overview

This is a polyclonal anti-PPP2R2B antibody raised in Rabbit, conjugated with FITC. Designed to detect PPP2R2B in Human, supporting researchers in signal transduction contexts.

Key elements and design rationale

• Immunogen: Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform protein (1-200AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
• Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
• Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
• Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
• Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
• FITC conjugate: Direct fluorescence (Ex ~490 nm / Em ~525 nm). Susceptible to photobleaching — use antifade reagents; protect from light.

Biological background

PPP2R2B (also known as 2ABB_HUMAN, B55 beta, Beta isoform of regulatory subunit B55 protein phosphatase 2, MGC24888, PP2A B55beta, PP2A PR55B, PP2A subunit B B beta isoform, PP2A subunit B B55 beta isoform, PP2A subunit B isoform B55 beta, PP2A subunit B isoform B55-beta, PP2A subunit B isoform beta, PP2A subunit B isoform PR55 beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2 beta, PP2A subunit B isoform R2-beta, PP2A subunit B PR55 beta isoform, PP2A subunit B R2 beta isoform, Ppp2r2b, PR2AB beta, PR2AB55 beta, PR2APR55 beta, PR52B, PR55 beta, Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) beta isoform, Protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform, Protein phosphatase 2 regulatory subunit B, Protein phosphatase 2 regulatory subunit B beta isoform, SCA 12, SCA12, Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, Serine/threonine protein phosphatase 2A neuronal isoform, Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of PPP2R2B biology relevant to signal transduction.

Common research applications

• Refer to the product datasheet for confirmed application data.

Notes for experimental interpretation

• Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
• Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
• Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
• Species reactivity: Confirmed for Human; extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (11108487)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.