| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PPP2R5E was used as the immunogen for the PPP2R5E antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPP2R5E Antibody / Serine threonine protein phosphatase 2A regulatory subunit B epsilon is a anti-PPP2R5E Rabbit antibody Recombinant Rabbit Monoclonal clone 30P58 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: PPP2R5E
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P58, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
PP2A holoenzymes consist of a scaffolding subunit, a catalytic subunit, and one of multiple regulatory subunits. PPP2R5E directs PP2A activity to specific substrates at centrosomes, kinetochores, and other cellular structures. Research using PPP2R5E antibody has shown that this regulatory subunit influences chromosome segregation, spindle formation, and checkpoint activation. These roles make it critical in maintaining genome stability and proper mitotic progression.
In cancer biology, PP2A acts as a tumor suppressor, and its inactivation contributes to oncogenic signaling. Altered expression of regulatory subunits such as PPP2R5E can impair PP2A function, enhancing signaling through pathways such as PI3K AKT and MAPK. Research with PPP2R5E antibody has demonstrated that loss of this subunit promotes tumorigenesis and contributes to chemotherapy resistance. Conversely, restoration of PP2A activity has been explored as a therapeutic strategy in malignancy, highlighting the importance of PPP2R5E in cancer control.
Beyond cancer, PPP2R5E has roles in neurodegeneration and cardiovascular disease. Studies using PPP2R5E antibody have linked PP2A dysfunction to tau hyperphosphorylation and Alzheimer disease pathology. In the cardiovascular system, PP2A regulatory subunits contribute to signaling in heart muscle contraction and vascular function. These findings underscore the broad physiological impact of PPP2R5E.
PPP2R5E antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting measures expression across proliferative and neuronal tissues, immunohistochemistry highlights nuclear localization, and immunoprecipitation isolates PP2A complexes containing PPP2R5E. These applications demonstrate the versatility of PPP2R5E antibody in molecular biology and translational research.
By supplying validated PPP2R5E antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
