| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors comprising three subtypes: PPARα, PPARδ (PPARβ/δ), and PPARγ. Each binds as a heterodimer with the retinoid X receptor to PPAR response elements (PPREs) in target gene promoters, where fatty acids and lipid-derived ligands switch the complex from a repressive to an activating state. Collectively, PPARs are central regulators of lipid and glucose metabolism, fatty acid oxidation, adipocyte differentiation, energy homeostasis, and inflammation. Because of these roles, PPAR signaling is closely tied to obesity, type 2 diabetes, and dyslipidemia, and PPARs are important drug targets in metabolic disease.
Product Description & Applications
The PPRE Reporter Lentivirus (Pan) is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of pan-PPAR (PPARα, PPARδ, PPARγ) activity in human or mouse cells. Tandem consensus PPAR response elements drive expression of a reporter (GFP, RFP, firefly luciferase, or Renilla luciferase), enabling quantitative monitoring of PPAR-mediated transcription in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study lipid and glucose metabolism, characterize PPAR ligands, and screen agonists and antagonists across the PPAR family. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
