| Field | Specification |
|---|---|
| Mfr No | |
| Formulation | |
| Immunogen | Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein. |
| Product Type | |
| Storage | |
| UniProt # |
Scientific Background
PRAS40 (Proline-rich AKT1 substrate 1, AKT1S1) is a direct AKT substrate and an inhibitory component of mTORC1. AKT-mediated phosphorylation of PRAS40 at Thr246 relieves mTORC1 inhibition, linking PI3K/AKT signalling to downstream mTOR-dependent cell growth, protein synthesis, and autophagy. PRAS40 is a key node in oncogenic PI3K–AKT–mTOR signalling.
Product Description
Blocking / Competition PeptideThis synthetic peptide corresponds to the immunogen sequence used to raise the matched St John's Laboratory antibody. It is supplied for immunodepletion and immunocompetition experiments to confirm antibody binding specificity. Pre-incubating the antibody with this peptide before immunoassay use abolishes or substantially reduces target-specific signal.
Product Specifications
| UniProt ID | Q96B36 |
|---|---|
| Immunogen | Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein. |
| Immunogen Region (aa) | 160-210 |
| Formulation | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Applications | Immunodepletion, Immunocompetition |
Recommended Protocol
Incubate the paired antibody (at working concentration) with a 5–10 molar excess of this peptide for 30–60 minutes at 4°C or room temperature. Centrifuge briefly if any precipitate forms, then use the pre-absorbed antibody in parallel with an unblocked control in the same experiment. Abolition or substantial reduction of signal confirms antibody specificity to the target epitope.
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
A blocking (or competition) peptide corresponds to the immunogen used to raise the paired antibody. Pre-incubating the antibody with this peptide allows it to compete with native antigen for antibody binding, abolishing or substantially reducing the specific signal in your immunoassay. This confirms that the signal observed is driven by target-specific antibody binding rather than non-specific interactions.
This peptide is matched to its corresponding St John’s Laboratory antibody. Refer to the product datasheet for the paired antibody catalog number.
Incubate the paired antibody at its working concentration with a 5–10 molar excess of this peptide for 30–60 minutes at 4°C or room temperature with gentle mixing. Centrifuge briefly if precipitate forms. Run this pre-absorbed antibody in parallel with an unblocked control in the same experiment and compare the signals to assess antibody specificity.
Store at-20°C for long term storage. Avoid freeze-thaw cycles.. Prepare small working aliquots upon receipt to avoid repeated freeze-thaw of the bulk stock.
This peptide is validated specifically for immunodepletion and immunocompetition assays. It is not validated as an immunogen for antibody production or as an ELISA standard unless stated on the datasheet. Contact BioHippo technical support for guidance on application-specific use outside this scope.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput screening or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Extended QC documentation: Additional purity methods, endotoxin testing, or bioactivity assay data can be requested for GMP-adjacent applications.
Please contact BioHippo customer support to discuss your specific requirements. Customisation availability and lead times vary by product and supplier.
