| Field | Specification |
|---|---|
| Mfr No | |
| Formulation | |
| Product Type | |
| Storage | |
| UniProt # |
Scientific Background
PRAS40 (Proline-rich AKT1 substrate 1, AKT1S1) is a direct AKT substrate and an inhibitory component of mTORC1. AKT-mediated phosphorylation of PRAS40 at Thr246 relieves mTORC1 inhibition, linking PI3K/AKT signalling to downstream mTOR-dependent cell growth, protein synthesis, and autophagy. PRAS40 is a key node in oncogenic PI3K–AKT–mTOR signalling.
Product Description
Positive Control PeptideThis positive control is supplied as 100 µL of pre-denatured protein in Laemmli SDS-PAGE sample buffer (SDS, glycerol, Tris pH 6.8, beta-mercaptoethanol). It is designed for direct loading onto SDS-PAGE gels without further preparation, enabling consistent Western Blot positive controls in the same run as experimental samples.
Product Specifications
| UniProt ID | Q96B36 |
|---|---|
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli s buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is empirically adjusted to get a clear band with signal to noise ratio for its corresponding antibody. |
| Recommended Dilution | WB: 1:500 |
| Storage | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Application | Western Blot (WB) |
Usage Notes
Load 5–10 µL per lane (or as directed by the paired antibody datasheet). Centrifuge the vial briefly before opening. Divide into single-use aliquots after first use to minimise freeze-thaw degradation.
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
A positive control peptide is a pre-denatured protein sample in SDS-PAGE loading buffer, designed to be loaded alongside experimental samples to confirm that all Western Blot steps — gel, transfer, blocking, antibody incubation, and detection — are functioning correctly. A clearly visible band at the expected molecular weight validates the entire workflow before interpreting experimental results.
This product is supplied ready to use. No reconstitution, heating, or buffer preparation is required. Centrifuge the vial briefly, then pipette 5–10 µL per lane and load directly onto your SDS-PAGE gel alongside experimental samples.
Store at −20°C immediately upon receipt for long-term stability. Store at-20°C for long term storage. Avoid freeze-thaw cycles.. Divide into single-use aliquots upon first opening to minimise freeze-thaw degradation. Do not store thawed product at 4°C for more than 48 hours.
Yes. Loading this control in a dedicated lane and probing with the corresponding antibody confirms detection under your experimental conditions. Note that the sample is denatured, so it specifically validates Western Blot conditions. Results may not predict antibody performance in native-condition assays (immunoprecipitation, immunofluorescence, IHC).
The UniProt accession is Q96B36, available at uniprot.org to verify canonical sequence, isoform data, and expression information.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput screening or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Extended QC documentation: Additional purity methods, endotoxin testing, or bioactivity assay data can be requested for GMP-adjacent applications.
Please contact BioHippo customer support to discuss your specific requirements. Customisation availability and lead times vary by product and supplier.
