| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Proline Carboxypeptidase, Angiotensinase C, PCP, Lysosomal Pro-X carboxypeptidase |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
PrCP is a lysosomal prolylcarboxypeptidase that cleaves C-terminal amino acids linked to proline. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. PrCPs role in angiotension II cleavage, which regulates blood pressure and electrolyte balance, suggests that PrCP may be involved in hypertension.
Product Description
Mammalian (HEK293) ExpressionRecombinant PrCP Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P42785 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 31-496 |
| Affinity Tag | C-terminal His-tag |
| Molecular Weight | 53 kDa |
| Formulation | 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 200 mM imida… |
| Storage | Stable at least 6 months from date of receipt, when stored as directed |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 31-496. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for the cleavage of C-terminal amino acids linked to proline in peptides, for example, angiotension II, III and des-Arg9-bradykinin. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
Stable at least 6 months from date of receipt, when stored as directed Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Tan, F., et al.
- J Biol Chem 268 (22): 16631-8.
- Yang, H.Y., et al.
- Nature 218 (5148): 1224-6.
- Wang, L., et al.
- Am. J. Obstet. Gynecol. 195 (1): 162-71.
