Preparative Protein Folding Column Set

SKU:BHT16300170
Overview
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Column sets for preparative folding of proteins from inclusion bodies under one of 10 conditions selected with the Spin-Column Protein Folding Screen Kit. Choose the column number (1–10) and the scale: 4 large-scale columns or 10 small-scale spin columns.
Condition Selection 10 column numbers
Format Large-scale or Small-scale
Shipping Cold, Not Frozen
Options selector
Catalog no. Scale Column #
SFC01 Small-scale (10 spin columns)
PFC01 Large-scale (4 columns)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Scale × Column #: Small-scale (10 spin columns) or Large-scale (4 columns), each for Column #1 to #10 (20 options)
  • Difference between options: Column # selects the folding condition (the same numbering as the 10 columns of the Screen Kit). Large-scale sets contain 4 columns with 5.4 ml Solution A, 8.4 ml Solution B and 15.4 ml Solution C, for folding 10–20 mg of denatured protein; small-scale sets contain 10 spin columns with 160 µl Solution A and 600 µl Solution B.
  • Select the scale and the column number from your screen before adding to cart.
  • Before this step: find the best column number with the Spin-Column Protein Folding Screen Kit (SFC01-10).
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped cold (not frozen).
  • Upon receipt: unpack promptly.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Protein Refolding
Storage Please contact us to confirm storage conditions.
Shipping Shipped cold (not frozen).
Catalog no. (Mfr.) SFC01 | SFC02 | SFC03 | SFC04 | SFC05 | SFC06 | SFC07 | SFC08 | SFC09 | SFC10 | PFC01 | PFC02 | PFC03 | PFC04 | PFC05 | PFC06 | PFC07 | PFC08 | PFC09 | PFC10
Main SKU BHT16300170
Protein Folding

Product Overview

The Preparative Protein Folding Column Set is used to fold proteins from solubilized inclusion bodies under one selected condition, after the best condition has been found with the Spin-Column Protein Folding Screen Kit. Each column number corresponds to the column with the same number in the Screen Kit.

Two scales are available for each of the 10 conditions: a small-scale set with 10 identical spin columns and a large-scale set with 4 columns for folding 10–20 mg of denatured protein.

Product Description

Component Large-scale (4 columns) Small-scale (10 spin columns)
Catalog # PFC01 to PFC10 SFC01 to SFC10
Columns 4 columns of the selected number 10 spin columns of the selected number
Solution A 5.4 ml 160 µl
Solution B 8.4 ml 600 µl
Solution C 15.4 ml —
Protein per set 10–20 mg denatured protein —

Specifications

Folding conditions 10 (Column #1–10)
Scales Large-scale (4 columns), Small-scale (10 spin columns)
Catalog # PFC01 to PFC10 (large-scale); SFC01 to SFC10 (small-scale)
Storage Please contact us to confirm storage conditions.
Shipping Shipped cold (not frozen).

The ProFoldin protocol “Spin-Column Protein Folding Screen” is linked under Documents.

Notes

  • Catalog #s SFC01 to SFC10 are the small-scale sets on this page, each with 10 identical spin columns of one selected condition. Catalog # SFC01-10 is a different product: the Spin-Column Protein Folding Screen Kit, which contains all 10 conditions (one column each) for screening.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.
Q.What is the difference between the large-scale and small-scale options?
A.Large-scale sets (PFC01 to PFC10) contain 4 columns with 5.4 ml Solution A, 8.4 ml Solution B and 15.4 ml Solution C, for 10–20 mg of denatured protein. Small-scale sets (SFC01 to SFC10) contain 10 spin columns with 160 µl Solution A and 600 µl Solution B.
Q.Which column number should I order?
A.Use the number of the column that gave the best result in the Spin-Column Protein Folding Screen Kit; the 10 numbers are the same 10 folding conditions.
Q.Is SFC01-10 one of these sets?
A.No. SFC01-10 is the Spin-Column Protein Folding Screen Kit with all 10 conditions for screening. SFC01 to SFC10 on this page are small-scale sets, each with 10 identical columns of one selected condition.
Q.Is a protocol available?
A.Yes. The ProFoldin protocol “Spin-Column Protein Folding Screen” is linked under Documents.
Q.How is it shipped and stored?
A.Shipped cold (not frozen). Please contact us to confirm storage conditions.

Can’t find the exact format you need? We can help you source or customize this Preparative Protein Folding Column Set — including larger pack sizes or bulk orders. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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