PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD Recombinant Monoclonal Antibody

Overview

This is a monoclonal anti-PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD antibody raised in Rabbit, with confirmed utility in ELISA, WB, IHC, FC. It is designed to detect PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD protein in Human, Mouse, Rat and supports researchers working in others contexts.

Key elements and design rationale

• Immunogen: A synthesized peptide from human PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.

Biological background

PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD (also referred to as KPCG_HUMAN antibody, MGC57564 antibody, OTTHUMP00000067291 antibody, PKC-gamma antibody, PKCC antibody, PKCG antibody, PRKCG antibody, Protein kinase C gamma antibody, Protein kinase C gamma polypeptide antibody, Protein kinase C gamma type antibody, Protein kinase C, gamma antibody, SCA 14 antibody, SCA14 antibody, KPCB_HUMAN antibody, PKC Beta antibody, PKC-B antibody, PKC-beta antibody, PKCB antibody, Prkcb antibody, PRKCB II antibody, PRKCB2 antibody, Protein kinase C beta antibody, Protein kinase C beta type antibody, KPCA_HUMAN antibody, PKC alpha antibody, PKC beta antibody, PKC delta antibody, PKC epsilon antibody, PKC gamma antibody, PKC zeta antibody, PKC-A antibody, PKC-alpha antibody, PKC2 antibody, PKCA antibody, PKCB antibody, PKCD antibody, PKCE antibody, PKCG antibody, PRKCA antibody, PRKCB antibody, PRKCB1 antibody, PRKCB2 antibody, PRKCD antibody, PRKCE antibody, PRKCG antibody, PRKCZ antibody, Protein kinase C alpha antibody, Protein kinase C alpha type antibody, Protein kinase C antibody, Protein kinase C beta antibody, Protein kinase C delta antibody, Protein kinase C epsilon antibody, Protein kinase C gamma antibody, Protein kinase C zeta antibody, KPCE_HUMAN antibody, MGC125656 antibody, MGC125657 antibody, nPKC epsilon antibody, nPKC-epsilon antibody, PKCE antibody, Pkcea antibody, PRKCE antibody, Protein kinase C epsilon antibody, Protein kinase C epsilon type antibody, 14-3-3-zetaisoform antibody, AI098070 antibody, aPKCzeta antibody, C80388 antibody, EC 2.7.11.13 antibody, KPCZ_HUMAN antibody, nPKC zeta antibody, nPKC-zeta antibody, OTTHUMP00000001368 antibody, OTTHUMP00000044160 antibody, PKC 2 antibody, PKC ZETA antibody, PKC2 antibody, Pkcz antibody, PKCZETA antibody, PKM-zeta, included antibody, PRKCZ antibody, Protein kinase C zeta antibody, Protein kinase C zeta form antibody, Protein kinase C zeta type antibody, r14-3-3 antibody, R74924 antibody, zetaPKC antibody, CVID9 antibody, D14Ertd420e antibody, Kinase PKC delta antibody, KPCD antibody, KPCD_HUMAN antibody, MAY 1 antibody, MAY1 antibody, MGC49908 antibody, nPKC delta antibody, nPKC-delta antibody, PCKd antibody, PKC d antibody, PKC delta antibody, PKCD antibody, PKCdelta antibody, PRKC D antibody, PRKC delta antibody, Prkcd antibody, Protein Kinase C delta antibody, Protein kinase C delta type antibody, Protein kinase C delta VIII antibody, Protein Kinase Cdelta antibody, Tyrosine protein kinase PRKCD antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD biology in others.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
• WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
• IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
• FC: Applicable for FC-based detection; refer to product documentation for optimized conditions.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation.

This product is a recombinant monoclonal antibody expressed in HEK293F cells.

• Datasheet (12945967)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.