| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Biological Activity | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Escherichia Coli. |
| Species | |
| Storage | |
| Target |
Product Description
Protein Arginine Methyltransferase 3 Human Recombinant
Overview
PRMT1 Methylates (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones). Methylates SUPT5H.The PRMT1 protein functions as a histone methyltransferase specific for H4.PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer.PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation.PRMT1 arginine-methylates MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response.PRMT1 plays a post-translationally part in regulating the transcriptional activity.PRMT1is found predominantly in the cytoplasma though a fraction of PRMT1 is located in the nucleus.
Biological Activity
Specific activity is defined as the amount of enzyme that transfer 1.0nmole of methyl group per minute at 37C and was found to be > 30nmol/min/mg.
Specifications
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
|---|---|
| Formulation | The PRMT1 solution (1mg/ml) contains 40mM Tris-HCl pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT and 40% glycerol. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | Escherichia Coli. |
