| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
PROP1 (Prophet of Pit-1) is a paired-like homeodomain transcription factor expressed transiently during early pituitary development. It is required for the differentiation of the anterior pituitary cell lineages and, notably, for activation of the PIT-1 (POU1F1) gene, placing it upstream of the regulatory cascade that establishes hormone-producing cell types. Loss-of-function mutations in PROP1 are a leading genetic cause of combined pituitary hormone deficiency, affecting production of growth hormone, thyroid-stimulating hormone, luteinizing hormone, follicle-stimulating hormone, and prolactin. Conversely, elevated PROP1 expression has been linked to proliferation of pituitary cell types. Reporters based on PROP1 response elements allow study of this developmental transcription factor.
Product Description & Applications
The PROP1 Reporter Lentivirus is a transcription-factor reporter system for detecting PROP1 transcriptional activity in mammalian cells. It uses tandem repeats of PROP1 response elements derived from the human PIT-1 promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter enable generation of stable polyclonal reporter cell lines with readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable lentiviral integration provides consistent, representative reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research into pituitary development and combined pituitary hormone deficiency.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the PROP1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
