Protein A Plates

Protein A Plates are specifically designed to capture IgG, either directly or as part of antibody/antigen complexes. The Protein A surface offers an alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA and other plate-based assays. Protein A binds specifically to the Fc region of immunoglobulins from various mammalian species, leaving the F(ab)2 binding sites free for efficient interaction with epitopes.

The Protein A Coated 96-Well Plates enable specific and sterically oriented binding of IgG, making them ideal for a variety of applications. These include the separation of IgG from other immunoglobulins or contaminants, isolation of antigen-antibody complexes, and the analysis and isolation of fusion proteins. Additionally, the plates can be used for the detection and identification of red cell antibodies, particularly when using U-bottom plates, providing a versatile tool for advanced immunoassays and protein analysis.

Key Features

• Protein A surface: Captures IgG, either directly or as part of antibody/antigen complexes.
• Specific and sterically oriented binding of IgG
• Separation of IgG from other immunoglobulins or contaminants
• Isolation of antigen-antibody complexes
• Analysis and isolation of fusion proteins
• Detection and identification of red cell antibodies (applicable on U-bottom plates)
• Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
• Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
• Wells design: flat bottom.
• Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
• Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
  • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
• Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
• Alphanumeric Coding: For easy and accurate well identification during experiments.
• Recommended working volume: of 50 to 100 μl
• Packaging: Each coated plate is packed in a single barrier bag with desiccant
• Unit: Contains 5 plates
• Minimum order: 10 plates.
• Ready to use

Key Benefits

• Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
  • The mould design provides optical quality, important to reduce the background signal.
  • The rim protects the external face of the bottom from scratches
• Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
• Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
• Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
• Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

• Specific and sterically oriented binding of IgG
• Separation of IgG from other immunoglobulins or contaminants
• Isolation of antigen-antibody complexes
• Analysis and isolation of fusion proteins
• ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
• Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
• Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
• Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.