| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
Protein G Plates are designed for the efficient capture of IgG, whether applied directly or as part of an antibody/antigen complex. The Protein G surface provides an alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA and other plate-based assay techniques. Protein G binds specifically to the Fc region of immunoglobulins from various mammalian species, ensuring that the F(ab)2 binding sites remain free for optimal interaction with epitopes.
Protein G Coated Plates are used in a variety of applications, including the specific and sterically oriented binding of IgG, ensuring that the antibody is properly aligned for maximum binding efficiency. They are also ideal for the separation of IgG from other immunoglobulins, as well as the isolation of antigen-antibody complexes. Additionally, these plates are highly effective for the isolation and analysis of fusion proteins, making them a versatile tool for advanced immunoassays and protein studies.
Key Features
- Protein G surface: It binds a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD
- Specific and sterically oriented binding of IgG
- Separation of IgG from other immunoglobulins
- Isolation of antigen-antibody complexes
- Analysis and isolation of fusion proteins
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
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Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 50 to 100 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
Key Benefits
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Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
Applications
- Specific and sterically oriented binding of IgG
- Separation of IgG from other immunoglobulins
- Isolation of antigen-antibody complexes
- Analysis and isolation of fusion proteins
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
