| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Tbp7 was used as the immunogen for the PSMC4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMC4 Antibody / 26S proteasome regulatory subunit 6B / TBP-7 is a anti-PSMC4 Rabbit antibody Recombinant Rabbit Monoclonal clone 31P63 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PSMC4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31P63, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
The 26S proteasome consists of a 20S catalytic core and a 19S regulatory complex. PSMC4 is one of six ATPase subunits within the base of the 19S regulator. These ATPases act as a molecular motor, unfolding ubiquitinated substrates and translocating them into the catalytic chamber for degradation. The role of PSMC4 is critical for energy-dependent substrate processing. Detection with PSMC4 antibody supports studies on proteasome assembly, activity, and substrate specificity.
Dysfunction of proteasome regulation has been linked to neurodegenerative diseases, cancer, and autoimmune disorders. Mutations or altered expression of proteasome subunits, including PSMC4, can disrupt proteostasis and lead to the accumulation of toxic protein aggregates. PSMC4 antibody has therefore been used to examine proteasome activity in conditions such as Parkinson disease, Huntington disease, and multiple myeloma. In cancer biology, changes in proteasome function influence cell proliferation and apoptosis, making it a therapeutic target.
PSMC4 antibody is suitable for use in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting reveals subunit expression within the proteasome complex, while immunohistochemistry highlights localization in tissues with high proteolytic activity, such as liver and brain. Immunoprecipitation using PSMC4 antibody aids in proteasome purification and analysis of protein-protein interactions.
Researchers studying pathways of ubiquitin-mediated degradation, antigen presentation, and stress responses rely on antibodies such as PSMC4 antibody to track proteasome composition and function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
