| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PUF-A/KIAA0020/PUM3 recombinant protein (Position: Q318-K631) was used as the immunogen for the PUM3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PUM3 Antibody / Pumilio 3 is a anti-PUM3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PUM3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PUM3 contains eight PUF RNA-binding domains arranged in a crescent-shaped architecture that recognizes conserved 5'-UGUANAUA-3' motifs in RNA targets. It binds ribosomal RNA precursors and ribosome assembly factors, influencing the processing of 28S and 5.8S rRNA. Depletion of PUM3 results in rRNA maturation defects, altered nucleolar morphology, and reduced ribosome production. Beyond ribosome biogenesis, PUM3 may also modulate mRNA stability and translation through interactions with additional RNA-binding proteins and components of the exosome complex.
The PUM3 antibody is used in molecular biology, RNA regulation, and ribosome assembly research to detect Pumilio homolog 3 protein and analyze its cellular localization. Western blot analysis identifies a 74 kilodalton band, while immunofluorescence reveals prominent nucleolar staining overlapping with fibrillarin. Because of its role in nucleolar RNA metabolism, this antibody provides a tool to study gene expression regulation at the post-transcriptional level.
Recent studies suggest that PUM3 interacts with factors controlling RNA stability and surveillance, positioning it as a regulator of RNA quality control. The PUM3 antibody facilitates the exploration of these processes and supports studies of nucleolar function, gene expression, and RNA-protein network organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
