| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCDC139; Coiled coil domain containing 139; Coiled coil domain containing protein 139; Coiled-coil domain-containing protein 139; DOBI; FLJ32312; MGC126729; Pseudouridine synthase 10; Pseudouridylate synthase 10; Psi55 synthase; pus10; PUS10_HUMAN; Putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA pseudouridylate synthase; tRNA uridine isomerase; tRNA-uridine isomerase |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide of Human PUS10 |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-PUS10 antibody raised in Rabbit, with confirmed utility in ELISA, WB, IHC. It is designed to detect PUS10 protein in Human, Mouse and supports researchers working in epigenetics and nuclear signaling contexts.
Key elements and design rationale
- Immunogen: Synthetic peptide of Human PUS10 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
PUS10 (also referred to as CCDC139, Coiled coil domain containing 139, Coiled coil domain containing protein 139, Coiled-coil domain-containing protein 139, DOBI, FLJ32312, MGC126729, Pseudouridine synthase 10, Pseudouridylate synthase 10, Psi55 synthase, pus10, PUS10_HUMAN, Putative tRNA pseudouridine synthase Pus10, tRNA pseudouridine 55 synthase, tRNA pseudouridylate synthase, tRNA uridine isomerase, tRNA-uridine isomerase) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of PUS10 biology in epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
PUS10 is a protein target in Human, Mouse biology. This polyclonal antibody raised in Rabbit is designed to detect PUS10 in ELISA, WB, IHC applications, with IgG isotype.
Reported reactive against Human, Mouse. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known PUS10-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
