| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
PXR (Pregnane X Receptor, NR1I2, also called SXR) is a ligand-activated nuclear receptor that serves as a master regulator of xenobiotic and drug metabolism. Activated by a broad range of structurally diverse compounds, PXR heterodimerizes with the retinoid X receptor and binds PXR response elements (PXRE) in the promoters of genes encoding drug-metabolizing enzymes and transporters, most notably the cytochrome P450 enzyme CYP3A4. PXR is highly expressed in liver and intestine, where it coordinates the body's defense against potentially harmful chemicals. Because PXR governs drug clearance and underlies many drug-drug interactions, it is a key target in pharmacology and toxicology research.
Product Description & Applications
The PXRE Reporter Lentivirus is a transcription-factor reporter system for detecting PXR-mediated transcriptional activity in mammalian cells. The construct uses tandem repeats of the PXR response element from the CYP3A4 promoter, arranged in an ER6 configuration, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, so the readout reflects activation by PXR/RXR heterodimers. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on xenobiotic metabolism, CYP3A4 induction, and drug-drug interaction screening.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, RFP + Firefly Luc, BFP2, Gaussia Luc, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the Pregnane X receptor (PXR) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
