| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
Direct assay of acetoacetate in serum, plasma, urine and other biological samples. Sensitive and accurate. Linear detection range of 0.012 to 1 mM (1.2 - 100 nmole/well) for acetoacetate in 96-well plate assay. Convenient. No sample. The assay uses OD550nm for signal readout. Compatible sample input includes Serum, plasma, urine, and other biological samples. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD550nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 12 μM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range of 0.012 to 1 mM (1.2 – 100 nmole/well) for acetoacetate in 96-well plate assay.
Additional feature notes highlight Convenient. No sample pretreatment needed. The procedure involves adding a single working reagent and reading the optical density at room temperature in kinetic mode; High-throughput. Can be automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of acetoacetate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ACETOACETATEis one of three ketone bodies produced in the liver mainly from oxidation of fatty acids and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism. Acetoacetate is an unstable ketone body that spontaneously decarboxylates to release carbon dioxide and acetone.Simple, direct and automation-ready procedures for measuring acetoacetate (AAT) are very desirable. BioAssay Systems’ QuantiChrom™ Acetoacetate assay is based on a reaction with Sodium Nitroprusside forming a product with absorbance at 550 nm.
Detection method
Colorimetric (OD 550 nm).
Detection limit and analytical sensitivity
Reported detection limit: 12 μM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify acetoacetate in serum, plasma, urine by OD550 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine handling.
- Monitor time-course or pre/post changes in serum, plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How stable is Acetoacetate?
Acetoacetate is an unstable ketone body that spontaneously breaks down to acetone and carbon dioxide, or is converted to 3-hydroxybutyrate, which is more stable. Because of this inherent instability, samples should be assayed fresh, or stored at -80°C and assayed shortly thereafter.
Do samples need to be deproteinated?
This kit can measure acetoacetate in the presence of proteins, but there is the potential for enzymatic conversion of acetoacetate to 3-hydroxybutyrate. To deproteinate samples, pass the sample through a 10 kDa NMWCO spin filter. For low levels of acetoacetate or in cases where you suspect matrix interference, it may be necessary to employ an acetoacetate spike, or internal standard, in the sample.
What is the normal concentration of acetoacetate in the blood?
The normal levels of acetoacetate in non-diabetic patients should be below 66 μM, but the levels of acetoacetate in a patient experiencing diabetic ketoacidosis can be considerably higher. Please keep in mind that this kit is for research use only, and not to be used for diagnostic purposes.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
