QuantiChrom™ Alcohol dehydrogenase Assay Kit

Overview

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism. The assay uses OD565nm for signal readout. Compatible sample input includes Biological (e.g. plasma, serum, urine, tissue, and culture media). Typical stated assay timing is 30 min.

Key elements and design rationale

• Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Biological (e.g. plasma, serum, urine, tissue, and culture media), which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 0.4 U/L for interpreting low-signal samples.
• Feature emphasis: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.

Additional feature notes highlight Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.

Biological background

This product is centered on measurement of alcohol dehydrogenase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase that catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the breakdown of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay System’s non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Detection method

Colorimetric (OD 565 nm).

Detection limit and analytical sensitivity

Reported detection limit: 0.4 U/L.

Procedures and timing

Stated procedure or timing information: 30 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.

Common research applications

• Quantify alcohol dehydrogenase in biological (plasma, serum, urine) by OD565 nm readout.
• Compare treatment or phenotype groups using matched biological (plasma, serum, urine) handling.
• Monitor time-course or pre/post changes in biological (plasma, serum, urine) across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.