| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Compounds that affect aldehyde dehydrogenase activity |
| Detection method | |
| Assay time | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For evaluation and high-throughput screen (HTS) of aldehyde dehydrogenase modulators. The assay uses OD565nm for signal readout. Compatible sample input includes Compounds that affect aldehyde dehydrogenase activity. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect aldehyde dehydrogenase activity, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 30 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Rapid and reliable. Can be completed in under an hour.
Additional feature notes highlight Homogenous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved; Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of aldehyde dehydrogenase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ALDEHYDE DEHYDROGENASES (ALDHs) are a superfamily of oxidoreductases that catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of aldehyde dehydrogenase have been linked to both alcoholism and alcohol sensitivity in people. Inhibitors of the enzyme have been used in cases to treat alcoholism in patients. Cancer stem cell populations also display a heightened activity of ALDH, making ALDH inhibition a promising anti-cancer therapy approach. BioAssay Systems QuantiChrom™ Aldehyde Dehydrogenase Inhibitor Screening Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH in turn reduces a formazan reagent into a colored product the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction. The percent inhibition of a test compound can be determined by comparing the activity of ALDH treated with a test compound to the activity of untreated ALDH.
Detection method
Colorimetric (OD 565 nm).
Procedures and timing
Stated procedure or timing information: 30 min.
Screening services
BioAssay Systems offers comprehensive Aldehyde Dehydrogenase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify aldehyde dehydrogenase inhibitor screening in compounds that affect aldehyde dehydrogenase by OD565 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect aldehyde dehydrogenase handling.
- Monitor time-course or pre/post changes in compounds that affect aldehyde dehydrogenase across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with ALDH or control inhibitors?
No, these are not included.
How do I store the kit?
This kit is shipped on ice. Upon receiving, please store the kit at -20°C.
What species of ALDH was the kit optimized for?
The kit was optimized for ALDH from from S. cerevisia (baker’s yeast). .
What if I am interested in testing inhibition of a species besides baker’s yeast?
We recommend that you experimentally determine the Km and then adjust the volume of substrate so that the final concentration of the substrate in the 100 µL Reaction Volume is near the Km.
What ALDH inhibitors were tested using this kit?
The kit was tested with Disulfiram (CAS Number 97-77-8).
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
