QuantiChrom™ α-L-Fucosidase Assay Kit

Overview

For quantitative determination of α-L-Fucosidase activity in biological samples. The assay uses OD405nm for signal readout. Compatible sample input includes Plasma, serum, tissue, cell lysate, etc. Typical stated assay timing is 20 min.

Key elements and design rationale

• Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Plasma, serum, tissue, cell lysate, etc, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 1 U/L for interpreting low-signal samples.
• Feature emphasis: High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L.

Additional feature notes highlight Homogeneous and simple procedure. A simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes; Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day. Available format information for this listing includes 100 Tests.

Biological background

This product is centered on measurement of α-l-fucosidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

α-L-FUCOSIDASE (AFU)is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian carcinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.BioAssay System’s non-radioactive, colorimetric AFU assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.

Detection method

Colorimetric (OD 405 nm).

Detection limit and analytical sensitivity

Reported detection limit: 1 U/L.

Procedures and timing

Stated procedure or timing information: 20 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Quantify α-l-fucosidase in plasma, serum, tissue, cell lysate by OD405 nm readout.
• Compare treatment or phenotype groups using matched plasma, serum, tissue, cell lysate handling.
• Monitor time-course or pre/post changes in plasma, serum, tissue, cell lysate across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.