| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples e.g. plasma, serum, tissue, and culture media |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of α-mannosidase enzyme activity. The assay uses OD405nm for signal readout. Compatible sample input includes Biological samples e.g. plasma, serum, tissue, and culture media. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples e.g. plasma, serum, tissue, and culture media, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1 U/L for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Linear detection range (10 µL sample): 1 to 250 U/L for a 10-minute reaction.
Additional feature notes highlight Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of α-mannosidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
α-MANNOSIDASE (AMA) is an enzyme that catalyzes the cleavage of the α form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children. BioAssay Systems’ non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.
Detection method
Colorimetric (OD 405 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1 U/L.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify α-mannosidase in biological samples e.g. plasma, serum, tissue by OD405 nm readout.
- Compare treatment or phenotype groups using matched biological samples e.g. plasma, serum, tissue handling.
- Monitor time-course or pre/post changes in biological samples e.g. plasma, serum, tissue across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
