| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Compounds that affect arginase activity |
| Detection method | |
| Assay time | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For evaluation and high-throughput screen (HTS) of arginase modulators. The assay uses OD430nm for signal readout. Compatible sample input includes Compounds that affect arginase activity. Typical stated assay timing is 1 hour 45 min.
Key elements and design rationale
- Readout format: OD430nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect arginase activity, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 1 hour 45 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system; Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).
Biological background
This product is centered on measurement of arginase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in the breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, important for protection against NH 3 toxicity and for cell growth and repair. Excessive arginase activity has been linked to cardiovascular diseases and also contributes to vascular structural problems and neural toxicity. Studies show that arginase inhibitors have been proven to be beneficial in cardiovascular and nervous system diseases. Simple, direct, and automation-ready procedures for measuring arginase inhibition are highly desirable in Research and Drug Discovery.BioAssay Systems’ arginase inhibitor screening kit provides a sensitive and convenient method to screen for arginase inhibitors. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample. Percent inhibition of a test compound can be determined by comparing the color intensity of the reaction preincubated with the test compound with the color intensity of an untreated control reaction
Detection method
Colorimetric (OD 430 nm).
Procedures and timing
Stated procedure or timing information: 1 hour 45 min.
Screening services
BioAssay Systems offers comprehensive Arginase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify arginase inhibitor screening in compounds that affect arginase activity by OD430 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect arginase activity handling.
- Monitor time-course or pre/post changes in compounds that affect arginase activity across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with arginase or control inhibitors?
No, these are not included.
How do I store the kit?
This kit is shipped at room temperature. Store the Arginine Buffer at -20°C, and other components at 2-8°C for long term storage.
What species of arginase was the kit optimized for?
The kit was optimized for Human Arginase I.
What if I am interested in testing inhibition of a species besides human?
We recommend that you experimentally determine the Km and then adjust the volume of substrate so that the final concentration of the substrate in the 250 µL Reaction Volume is near the Km.
What arginase inhibitors were tested using this kit?
ABH ammonium salt.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
