| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Compounds that affect ATPase/GTPase activity |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. The assay uses OD620nm for signal readout. Compatible sample input includes Compounds that affect ATPase/GTPase activity. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD620nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect ATPase/GTPase activity, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.007 U/L for interpreting low-signal samples.
- Feature emphasis: High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
Additional feature notes highlight Fast and convenient: single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes; Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Available format information for this listing includes 200 Tests.
Biological background
This product is centered on measurement of atpase/gtpase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. BioAssay Systems QuantiChrom™ ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 – 660 nm).
Detection method
Colorimetric (OD 620 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.007 U/L.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify atpase/gtpase in compounds that affect ATPase/GTPase activity by OD620 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect ATPase/GTPase activity handling.
- Monitor time-course or pre/post changes in compounds that affect ATPase/GTPase activity across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Discriminating the short-term action of root and foliar application of humic acids on plant growth: Emerging role of jasmonic acid
De Hita, D et al. (2020). Discriminating the short-term action of root and foliar application of humic acids on plant growth: Emerging role of jasmonic acid. Frontiers in Plant Science, 11, 493. Assay: ATPase Assay Kit in cucumber roots.
Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin
Gong, D. et al. (2018). Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin. Nature communications 9(1):3623. Assay: GTPase in human hPMCA1-NPTN and hPMCA1.
Aciduricity and acid tolerance mechanisms of Streptococcus anginosus
Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T., & Kimura, S. (2018). Aciduricity and acid tolerance mechanisms of Streptococcus anginosus. The Journal of general and applied microbiology, 2017-11. Assay: GTPase in Streptococcus anginosus NCTC 10713.
The major-effect quantitative trait locus Cs ARN 6
Xu, X., Ji, J., Xu, Q., Qi, X., Weng, Y., & Chen, X. (2018). The major-effect quantitative trait locus Cs ARN 6.1 encodes an AAA ATP ase domain-containing protein that is associated with waterlogging stress tolerance by promoting adventitious root formation. The Plant Journal, 93(5), 917-930. Assay: GTPase in E. coli pCzn1-460 vector.
Mechanism of ATP hydrolysis by the Zika virus helicase
Yang, X., Chen, C., Tian, H., Chi, H., Mu, Z., Zhang, T. & Ji, X. (2018). Mechanism of ATP hydrolysis by the Zika virus helicase. The FASEB Journal, 32(10), 5250-5257. Assay: GTPase in enzymes.
Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B
Zhou, J. et al. (2018). Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B. Journal of virology, 92(7), e02147-17. Assay: GTPase in porcine PK-15 cells.
A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1
Chen, K., Guo, L., Zhang, J., Chen, Q., Wang, K., Li, C. & Chen, D. (2017). A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1. Acta biomaterialia, 48, 215-226. Assay: GTPase in mice RanGAP1.
Structure of the human lipid exporter ABCA1
Qian, H., Zhao, X., Cao, P., Lei, J., Yan, N., & Gong, X. (2017). Structure of the human lipid exporter ABCA1. Cell, 169(7), 1228-1239. Assay: GTPase in human ABCA1.
RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly
Sheng, Y., Song, Y., Li, Z., Wang, Y., Lin, H., Cheng, H., & Zhou, R. (2017). RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly. Cell Death & Differentiation 25(5):918-934. Assay: GTPase in COS-7 and HeLa cells RAB37.
Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution
Sun, S. et al. (2017). Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution. Nature communications, 8, 16064. Assay: GTPase in Saccharomyces cerevisiae Vps4 proteins.
Multi-transgenic pigs for diabetes treatment
Ayares, D. (2016). Multi-transgenic pigs for diabetes treatment. U.S. Patent No. 9,339,519. Assay: GTPase in porcine fetal fibroblasts.
Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design
Cao, X., Li, Y., Jin, X., Li, Y., Guo, F., & Jin, T. (2016). Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design. Nucleic acids research 44(21):10505-10514. Assay: GTPase in ZIKV NS3 helicase.
Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis
Cho, J. H., Lee, J. H., Park, Y. K., Choi, M. N., & Kim, K. N. (2016). Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis. Frontiers in Plant Science 7, 1911. Assay: GTPase in E. coli TOC34 protein.
Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama
El-Shesheny, I., Hijaz, F., El-Hawary, I., Mesbah, I., & Killiny, N. (2016). Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 192, 28-37. Assay: GTPase in Diaphorina citri hsp 70.
Plant bacterial pathogen manipulates the energy metabolism of its insect vector
Killiny, N., Hijaz, F., Ebert, T. A., & Rogers, M. E. (2016). Plant bacterial pathogen manipulates the energy metabolism of its insect vector. Applied and Environmental Microbiology 83(5). pii: e03005-16. Assay: GTPase in Diaphorina citri.
Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore
Seyedmohammad, S., Fuentealba, N. A., Marriott, R. A., Goetze, T. A., Edwardson, J. M., Barrera, N. P., & Venter, H. (2016). Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore. Bioscience reports, 36(2), e00322. Assay: GTPase in Pseudomonas aeruginosa FeoB Transporter.
Structural basis of Zika virus helicase in recognizing its substrates
Tian, H. et al. (2016). Structural basis of Zika virus helicase in recognizing its substrates. Protein & cell, 7(8), 562-570. Assay: GTPase in ZIKV helicase172-617.
Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule
Yang, J. et al (2016). Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule. Nature communications, 7, 12103. Assay: GTPase in E. coli tubulin.
Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata
Bertol JW, et al (2011). Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata. Antiviral Res. 92(1):73-80. Assay: ATPase/GTPase in human, monkey Na(+)K(+)ATPase.
Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I)
Binder M, et al (2011). Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I). J Biol Chem. 286(31):27278-87. Assay: ATPase/GTPase in human purified protein.
Identification of an mRNP complex regulating tumorigenesis at the translational elongation step
Hussey, GS et al (2011). Identification of an mRNP complex regulating tumorigenesis at the translational elongation step. Mol Cell. 41(4):419-31. Assay: ATPase/GTPase in human GTPase.
Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation
Sheikha GA, et al (2011). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 26.5: 603-609. Assay: ATPase/GTPase in human Hsp90 inhibition.
Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation
Sheikha, GA et al (2010). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 2010 Dec 29. [Epub ahead of print]. Assay: ATPase/GTPase in human Hsp90 inhibition.
A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi
Suematsu T, et al (2010). A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi. Mol Microbiol. 75(6):1445-54. Assay: ATPase/GTPase in bacteria/human ATPase.
Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces
Hu WW, et al (2009). Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces. J Control Release. 135(3):250-8. Assay: ATPase/GTPase in ATPase extract.
Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin
Gong, D. et al. (2018). Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin. Nature communications 9(1):3623. Assay: GTPase in human hPMCA1-NPTN and hPMCA1.
Aciduricity and acid tolerance mechanisms of Streptococcus anginosus
Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T., & Kimura, S. (2018). Aciduricity and acid tolerance mechanisms of Streptococcus anginosus. The Journal of general and applied microbiology, 2017-11. Assay: GTPase in Streptococcus anginosus NCTC 10713.
The major-effect quantitative trait locus Cs ARN 6
Xu, X., Ji, J., Xu, Q., Qi, X., Weng, Y., & Chen, X. (2018). The major-effect quantitative trait locus Cs ARN 6.1 encodes an AAA ATP ase domain-containing protein that is associated with waterlogging stress tolerance by promoting adventitious root formation. The Plant Journal, 93(5), 917-930. Assay: GTPase in E. coli pCzn1-460 vector.
Mechanism of ATP hydrolysis by the Zika virus helicase
Yang, X., Chen, C., Tian, H., Chi, H., Mu, Z., Zhang, T. & Ji, X. (2018). Mechanism of ATP hydrolysis by the Zika virus helicase. The FASEB Journal, 32(10), 5250-5257. Assay: GTPase in enzymes.
Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B
Zhou, J. et al. (2018). Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B. Journal of virology, 92(7), e02147-17. Assay: GTPase in porcine PK-15 cells.
A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1
Chen, K., Guo, L., Zhang, J., Chen, Q., Wang, K., Li, C. & Chen, D. (2017). A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1. Acta biomaterialia, 48, 215-226. Assay: GTPase in mice RanGAP1.
Structure of the human lipid exporter ABCA1
Qian, H., Zhao, X., Cao, P., Lei, J., Yan, N., & Gong, X. (2017). Structure of the human lipid exporter ABCA1. Cell, 169(7), 1228-1239. Assay: GTPase in human ABCA1.
RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly
Sheng, Y., Song, Y., Li, Z., Wang, Y., Lin, H., Cheng, H., & Zhou, R. (2017). RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly. Cell Death & Differentiation 25(5):918-934. Assay: GTPase in COS-7 and HeLa cells RAB37.
Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution
Sun, S. et al. (2017). Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution. Nature communications, 8, 16064. Assay: GTPase in Saccharomyces cerevisiae Vps4 proteins.
Multi-transgenic pigs for diabetes treatment
Ayares, D. (2016). Multi-transgenic pigs for diabetes treatment. U.S. Patent No. 9,339,519. Assay: GTPase in porcine fetal fibroblasts.
Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design
Cao, X., Li, Y., Jin, X., Li, Y., Guo, F., & Jin, T. (2016). Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design. Nucleic acids research 44(21):10505-10514. Assay: GTPase in ZIKV NS3 helicase.
Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis
Cho, J. H., Lee, J. H., Park, Y. K., Choi, M. N., & Kim, K. N. (2016). Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis. Frontiers in Plant Science 7, 1911. Assay: GTPase in E. coli TOC34 protein.
Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama
El-Shesheny, I., Hijaz, F., El-Hawary, I., Mesbah, I., & Killiny, N. (2016). Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 192, 28-37. Assay: GTPase in Diaphorina citri hsp 70.
Plant bacterial pathogen manipulates the energy metabolism of its insect vector
Killiny, N., Hijaz, F., Ebert, T. A., & Rogers, M. E. (2016). Plant bacterial pathogen manipulates the energy metabolism of its insect vector. Applied and Environmental Microbiology 83(5). pii: e03005-16. Assay: GTPase in Diaphorina citri.
Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore
Seyedmohammad, S., Fuentealba, N. A., Marriott, R. A., Goetze, T. A., Edwardson, J. M., Barrera, N. P., & Venter, H. (2016). Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore. Bioscience reports, 36(2), e00322. Assay: GTPase in Pseudomonas aeruginosa FeoB Transporter.
Structural basis of Zika virus helicase in recognizing its substrates
Tian, H. et al. (2016). Structural basis of Zika virus helicase in recognizing its substrates. Protein & cell, 7(8), 562-570. Assay: GTPase in ZIKV helicase172-617.
Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule
Yang, J. et al (2016). Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule. Nature communications, 7, 12103. Assay: GTPase in E. coli tubulin.
Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata
Bertol JW, et al (2011). Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata. Antiviral Res. 92(1):73-80. Assay: ATPase/GTPase in human, monkey Na(+)K(+)ATPase.
Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I)
Binder M, et al (2011). Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I). J Biol Chem. 286(31):27278-87. Assay: ATPase/GTPase in human purified protein.
Identification of an mRNP complex regulating tumorigenesis at the translational elongation step
Hussey, GS et al (2011). Identification of an mRNP complex regulating tumorigenesis at the translational elongation step. Mol Cell. 41(4):419-31. Assay: ATPase/GTPase in human GTPase.
Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation
Sheikha GA, et al (2011). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 26.5: 603-609. Assay: ATPase/GTPase in human Hsp90 inhibition.
Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation
Sheikha, GA et al (2010). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 2010 Dec 29. [Epub ahead of print]. Assay: ATPase/GTPase in human Hsp90 inhibition.
A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi
Suematsu T, et al (2010). A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi. Mol Microbiol. 75(6):1445-54. Assay: ATPase/GTPase in bacteria/human ATPase.
Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces
Hu WW, et al (2009). Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces. J Control Release. 135(3):250-8. Assay: ATPase/GTPase in ATPase extract.
