| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, biological preparations |
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Overview
For quantitative determination of albumin and evaluation of drug effects on albumin metabolism. The assay uses OD610nm (BCP) for signal readout. Compatible sample input includes Serum, plasma, urine, biological preparations. Typical stated assay timing is 5 min.
Key elements and design rationale
- Readout format: OD610nm (BCP) supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, biological preparations, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.3 g/dL for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 20 µL samples. Detection range 0.3 – 5 g/dL (45 – 750 µM) albumin in a 96-well plate assay.
Additional feature notes highlight Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day; Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvet or 96-well plate assay. Available format information for this listing includes 250 Tests.
Biological background
This product is centered on measurement of bcp albumin within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Albuminis the most abundant plasma protein in humans. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including the maintenance of colloid osmotic pressure, and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, and magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct, and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Detection method
Colorimetric (OD 610 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.3 g/dL.
Procedures and timing
Stated procedure or timing information: 5 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify bcp albumin in serum, plasma, urine, biological preparations by OD610 nm (BCP) readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine, biological preparations handling.
- Monitor time-course or pre/post changes in serum, plasma, urine, biological preparations across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Your company sells two different kit (DIAG-250 and DIAP-250). What are the differences between them exactly? Is it just the wavelength of detection?
The two kits use different albumin specific dyes and hence the major difference lies in the sensitivity and wavelengths. Both assays have advantages and disadvantages. DIAG-250 (QuantiChrom BCG Albumin Assay Kit) uses bromcresol green, a dye that is more (about 2-fold) sensitive than bromcresol purple used in the BCP albumin assay. BCG is not absolutely specific for albumin, however, it also reacts with α1- and α2-globulins, and adds a positive bias. The BCP method, although more specific, underestimates albumin in certain samples, e.g. from patients undergoing hemodialysis. If your samples are sera or plasma, which have high albumin concentrations, I would suggest using the DIAP-250 assay kit (detection wavelength 610 nm). If the albumin concentration is low, DIAG-250 is the best choice (detection wavelength 620 nm).
Pooled plasma samples (EDTA) show high albumin concentrations with the bromcresol purple assay? What could be interfering with the assay?
There should be no significant differences between serum samples and plasma samples. Turbid plasma samples (fibrinogen) can cause high albumin readings. This can be adjusted for by blanking samples at OD 690 nm. BCP does not absorb at 690 nm, whereas the absorbance caused by turbididty is the same at 610 nm and 690 nm.
Since the concentration that I am measuring is very low, would it be reasonable to dilute the standard curve down to lower level so my sample is still within the curve?
Yes, as long as the standard curve is within the limits of the dynamic range, but this will not increase the sensitivity of the assay. To increase the sensitivity add a larger volume of sample and standard. For example, you could add 20 μL sample and 20 μL of diluted standards (instead of starting at 5 g/dL as shown in the standard protocol, start at 1 g/dL).
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Senescence-accelerated mouse prone 8 mice exhibit specific morphological changes in the small intestine during senescence and after pectin supplemented diet
Ben Othman, S., et al. (2020). Senescence-accelerated mouse prone 8 mice exhibit specific morphological changes in the small intestine during senescence and after pectin supplemented diet. Experimental Gerontology 142. Assay: Albumin in mouse serum.
Redistribution of labile plasma zinc during mild surgical stress in the rat
Kelly E, et al (2011). Redistribution of labile plasma zinc during mild surgical stress in the rat. Transl Res. 157(3):139-49. Assay: Albumin in rat plasma.
Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus
Olsen AS, et al (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair Regen. 18(5):532-42. Assay: Albumin in zebrafish blood.
Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)
Sharifuzzaman SM and Austin B (2010). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 108(6): 2162 – 2170. Assay: Albumin in fish serum.
Immunological investigation in the adenoid tissues from children with chronic rhinosinusitis
Shin SY et al (2009). Immunological investigation in the adenoid tissues from children with chronic rhinosinusitis. Otolaryngol Head Neck Surg. 141(1):91-6. Assay: Albumin in human tissue.
IgE response to staphylococcal enterotoxins in adenoid tissues from atopic children
Shin SY, et al (2009). IgE response to staphylococcal enterotoxins in adenoid tissues from atopic children. Laryngoscope. 119(1):171-5. Assay: Albumin in human serum.
Local production of total IgE and specific antibodies to the house dust mite in adenoid tissue
Shin SY, et al (2009). Local production of total IgE and specific antibodies to the house dust mite in adenoid tissue. Pediatr Allergy Immunol. 20(2):134-41. Assay: Albumin in human blood.
Anti-DNA antibody induction of protein kinase C phosphorylation and fibronectin synthesis in human and murine lupus and the effect of mycophenolic acid
Yung S, et al (2009). Anti-DNA antibody induction of protein kinase C phosphorylation and fibronectin synthesis in human and murine lupus and the effect of mycophenolic acid. Arthritis Rheum. 60(7):2071-82. Assay: Albumin in human urine.
Maier SM et al (2007) Proteinuria of Nonautoimmune Origin in Wild-type FVB/NJ Mice. Comp Med. 57(3) 255-266. Assay: Albu
Maier SM et al (2007) Proteinuria of Nonautoimmune Origin in Wild-type FVB/NJ Mice. Comp Med. 57(3) 255-266. Assay: Albumin in mouse urine and serum.
