| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological |
| Shipping | |
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| Storage |
Overview
For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism. The assay uses OD405nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 2 U/L for interpreting low-signal samples.
- Feature emphasis: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L.
Additional feature notes highlight Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of β-glucosidase activity within 20 minutes; Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of β-glucosidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
β-GLUCOSIDASEis a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Detection method
Colorimetric (OD 405 nm).
Detection limit and analytical sensitivity
Reported detection limit: 2 U/L.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify β-glucosidase in biological by OD405 nm readout.
- Compare treatment or phenotype groups using matched biological handling.
- Monitor time-course or pre/post changes in biological across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How to use of DBGD-100 assay kit for human β-glucosidase activity?
The β-glucosidase assay comes with a neutal assay buffer (60 mM KPi, pH 7.0) and is suitable for enzymes that show activity around pH 7. The product of the reaction develops an intense yellow color at high pH (9-11).
The human enzyme has a pH optimum at 5.5. It is advisable to carry out the reaction in a buffer with that pH. However, because the absorbance of the colored product at pH 5 is lower than at pH 7, you have to raise the pH before measuring the absorbance.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Increased α-synuclein oligomerization is associated with decreased activity of glucocerebrosidase in the aging human striatum and hippocampus
Yang, W et al (2020). Increased α-synuclein oligomerization is associated with decreased activity of glucocerebrosidase in the aging human striatum and hippocampus. Neuroscience Letters, 733, 135093. Assay: β-glucosidase in human.
α-Syn oligomers incubated with Parkinsons disease plasma promote neuron damage
Yang, W et al (2020). α-Syn oligomers incubated with Parkinsons disease plasma promote neuron damage. International Journal of Clinical and Experimental Pathology, 13(8), 1995-2008. Assay: β-glucosidase in human plasma.
Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines
Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Assay: beta-Glucosidase in human cells.
Identification of beta-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng
Renchinkhand, G et al (2015). Identification of beta-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Assay: beta-Glucosidase in Enterococcus faecalis bacteria.
Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach
Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: beta-Glucosidase in mammal cells.
Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins
Mallery SR, et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: beta-Glucosidase in human saliva.
