| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Blood, tissue and bacterial samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of β-glucuronidase enzyme activity in biological samples, e.g. blood, tissue and bacterial samples. The assay uses FL365/450nm for signal readout. Compatible sample input includes Blood, tissue and bacterial samples. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: FL365/450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Blood, tissue and bacterial samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 10 -4 U/L for interpreting low-signal samples.
- Feature emphasis: Highly Sensitive. The fluorimetric nature of the kit detects 1×10 -4 U/L to 8 U/L BG activity in 96 well plate with only 10 µL sample within 30 min.
Additional feature notes highlight Versatile. This assay has been validated to work with both animal and E. coli derived β-glucuronidase; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of β-glucuronidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
BETA-GLUCURONIDASE (BG) is an enzyme which catalyzes the hydrolysis of β-glucuronide bonds. In humans, β-glucuronidase catalyzes the hydrolysis of β-D-glucuronic acids from glycoproteins and mucopolysaccharides such as heparan sulfate. β-glucuronidase activity is important for drug metabolism as drugs are often conjugated to glucuronic acid to make for a more water soluble delivery system, relying on subsequent β-glucuronidase activity to release the active drug. Bilirubin clearance is also facilitated by glucuronidation. GUSB is often used as a reporter gene to track gene expression through β-glucuronidase activity. BioAssay Systems’ fluorimetric BG assay uses a β-D-glucuronide substrate which fluoresces upon hydrolysis with β-glucuronidase and is read at 365/450 nm.
Detection method
Fluorescent (FL 365/450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 10 -4 U/L.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify β-glucuronidase in blood, tissue and bacterial samples by FL365/450 nm readout.
- Compare treatment or phenotype groups using matched blood, tissue and bacterial samples handling.
- Monitor time-course or pre/post changes in blood, tissue and bacterial samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Will this kit detect β-glucuronidase from my organism?
This kit was optimized for mammalian BG, but also works with E.coli BG. The substrate is at pH 5.0 and should be suitable for enzymes with optimal pH in the range of 3-8. We recommend to run an intial test with 30 minute incubation and if results show low activity, extend the incubation to 60 or 90 minutes.
Can I run this assay in a black 384 well plate?
Yes, to use 384 well plate format simply divide all volumes by two, e.g. 5 µL sample or standard, 20 µL substrate, 7.5 µL stop reagent. Otherwise the protocol is the same.
Is sample background fluorescence an issue?
In our testing we did not see noticeable background from tissue lysates or serum samples. Generally samples do not fluoresce at the kit’s wavelength, or are diluted enough to not be an issue. In the rare case that samples do have significant background fluorescence we advise to include a sample blank that uses deionized water in lieu of substrate.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
