QuantiChrom™ Chloride Assay Kit

Overview

For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. The assay uses OD610nm for signal readout. Compatible sample input includes Biological, food, and environment. Typical stated assay timing is 5 min.

Key elements and design rationale

• Readout format: OD610nm supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Biological, food, and environment, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 0.7 mg/dL (0.2 mM) for interpreting low-signal samples.
• Feature emphasis: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl – in 96-well plate assay.

Additional feature notes highlight Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day; Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvet or 96-well plate assay. Available format information for this listing includes 250 Tests.

Biological background

This product is centered on measurement of chloride within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

Chlorideis the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure, and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis, and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis. Simple, direct, and automation-ready procedures for measuring chloride concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems chloride assay kit is designed to measure chloride directly in biological samples without any pretreatment. The improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color, measured at 610nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Detection method

Colorimetric (OD 610 nm).

Detection limit and analytical sensitivity

Reported detection limit: 0.7 mg/dL (0.2 mM).

Procedures and timing

Stated procedure or timing information: 5 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.

Common research applications

• Quantify chloride in biological, food, and environment by OD610 nm readout.
• Compare treatment or phenotype groups using matched biological, food, and environment handling.
• Monitor time-course or pre/post changes in biological, food, and environment across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.