| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Cell lysates, tissues, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
Direct assays of citrate synthase activity in cell lysates, tissues, and other biological samples. Linear detection range 0.50 to 120 U/L citrate synthase activity in 96-well plate assay. Simple and convenient. The procedure involves. The assay uses OD412nm for signal readout. Compatible sample input includes Cell lysates, tissues, and other biological samples. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD412nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Cell lysates, tissues, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.50 U/L for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range 0.50 to 120 U/L citrate synthase activity in 96-well plate assay.
Additional feature notes highlight Simple and convenient. The procedure involves adding a single working reagent and reading the optical density at 5 min and 10 min at room temperature; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of citrate synthase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CITRATE SYNTHASE(E.C. 2.3.3.1) is a pivotal enzyme that initiates the tricarboxylic acid (TCA) cycle. It catalyzes the condensation reaction between acetyl-CoA and oxaloacetic acid, yielding citric acid. Citrate synthase levels of cells and tissue are used as a guide in determining mitochondrial dysfunctions and cellular metabolism. High levels of citrate synthase activity indicate increased metabolic activity or cellular energy production. This can occur in situations where cells are actively metabolizing substrates for energy production, such as during periods of increased physical activity or in tissues with high energy demands, like muscle tissue. Conversely, low levels of citrate synthase activity may suggest reduced metabolic activity or energy production. This could be seen in situations where cellular metabolism is slowed down, such as during periods of rest or in conditions associated with mitochondrial dysfunction. Simple, direct and automation-ready procedures for measuring citrate synthase activity are very desirable. BioAssay Systems’ QuantiChrom™ Citrate Synthase Assay is based on an improved Ellman method, in which coenzyme A thiol produced by the action of citrate synthase forms a yellow color with 5,5′-dithiobis (2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
Detection method
Colorimetric (OD 412 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.50 U/L.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify citrate synthase in cell lysates, tissues by OD412 nm readout.
- Compare treatment or phenotype groups using matched cell lysates, tissues handling.
- Monitor time-course or pre/post changes in cell lysates, tissues across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this kit supply Citrate Synthase?
No, this kit is for the measurement of Citrate Synthase activity, so researchers must supply their own Citrate Synthase enzyme or sample specific to their needs.
What is the species origin of the Citrate Synthase used to develop this kit?
Citrate Synthase from E. coli was used to develop the kit, but the kit has been used to detect Citrate Synthase in variety of cell lines and tissue homogenates.
How long are the solubilized reagents good for?
Reagent A and Reagent B should be used within two (2) weeks of dissolution.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
