| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Aqueous samples |
| Shipping | |
| Species | |
| Storage |
Overview
Direct Assays: cobalt in aqueous samples. The assay uses OD485nm for signal readout. Compatible sample input includes Aqueous samples. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD485nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Aqueous samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1.4µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range from 1.4 µM to 200 µM cobalt ions in a 96-well plate assay.
Additional feature notes highlight Simple and high-throughput. The procedure involves the addition of a single working reagent with immediate color development. Can be readily automated as a high-throughput assay for thousands of samples per day; Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent stability. Cuvette or 96-well plate assay. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of cobalt within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Cobalt is an essential micronutrient for all multicellular organisms as the active center of cobalamin, such as vitamin B-12, which is essential for plants and animals. Cobalt is also a micronutrient for bacteria, algae, and fungi. Despite being an important micronutrient, excess cobalt in the environment can be deleterious to life leading to cardiomyopathy in humans and necrosis in plants.Simple, direct, and automation-ready procedures for measuring cobalt concentrations find wide applications in research and environmental monitoring. BioAssay Systems’ cobalt assay kit is designed to measure total cobalt directly in aqueous samples without any pretreatment. The intensity of the color, measured at 485nm, is directly proportional to the cobalt concentration in the sample.
Detection method
Colorimetric (OD 485 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1.4µM.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify cobalt in aqueous samples by OD485 nm readout.
- Compare treatment or phenotype groups using matched aqueous samples handling.
- Monitor time-course or pre/post changes in aqueous samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Do other ions interfere with the detection of Cobalt ions?
The metal ions Fe2+, Cu2+, Zn2+, Mn2+, Mg2+, Al3+, Mo2+, Pb2+ and W2+ exhibit less than 10% interference in this assay. Ni2+ concentrations >10% of Co2+ concentration should be avoided. Excessive Ca2+ may cause precipitation to occur.
Will increasing the incubation time with the Detection Reagent increase the sensitivity of the assay?
No, increasing the incubation time beyond about 15-20 minutes will only result in the formation of a precipitate. The color formation with Cobalt ion is almost instantaneous, so a longer incubation time is not required.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
