| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Food, beverages, and biological samples |
| Detection limit | |
| Detection method | |
| Plate format | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For quantitative determination of antioxidant capacity in food, beverages and biological samples. Sensitive and accurate. Detects 11 µM to 300 µM Trolox equivalents in 96-well plate. Stable reagent. DPPH in this kit is included as a powder. The assay uses OD517nm for signal readout. Compatible sample input includes Food, beverages, and biological samples. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD517nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food, beverages, and biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 11 μM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Detects 11 μM to 300 μM Trolox equivalents in 96-well plate.
Additional feature notes highlight Stable reagent. DPPH in this kit is included as a powder form that can be aliquoted to test as many or few wells as desired, or repeated testing over separate days, or weeks; Simple and high-throughput. This room temperature, homogenous, “mix-incubate-measure” assay can be run in as little as 10 minutes. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 120 Tests in 96-well plate.
Biological background
This product is centered on measurement of dpph antioxidant capacity within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
AnANTIOXIDANTis a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants, such as the small molecules glutathione and vitamins or the macromolecules catalase and glutathione peroxidase, protect the cells from damage by reactive oxygen species. Antioxidants are widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline.Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. BioAssay Systems’ improved antioxidant capacity assay is based on the reduction of DPPH by antioxidant molecules in a sample, which results in a drop in absorbance at 517 nm. The antioxidant capacity can be quantified and expressed using a Trolox standard.
Detection method
Colorimetric (OD 517 nm).
Detection limit and analytical sensitivity
Reported detection limit: 11 μM.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify dpph antioxidant capacity in food, beverages, and biological samples by OD517 nm readout.
- Compare treatment or phenotype groups using matched food, beverages, and biological samples handling.
- Monitor time-course or pre/post changes in food, beverages, and biological samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What units should I use to represent my results?
The default units for representing antioxidant capacity in DAOC-120 are trolox equivalent antioxidant capacity(TEAC) expressed in terms of μM trolox equivalents. This is calculated by plotting the delta OD for samples on the trolox standard curve and determining TEAC for your samples. Antioxidant capacity can also be expressed in terms of % inhibition of the DPPH OD517, where % inhibition = 100 x ((ODSTD0 – ODsample – ODsample blank) / ODSTD0). Antioxidant capacity can also be reported in terms of EC50, where you test several different dilutions of your sample and create a sample dilution curve. Then, using the dilution curve you can calculate the amount of sample that causes 50% DPPH inhibition.
Can I read at wavelengths other than 517 nm?
We recommend reading at 517 nm because it is the most sensitive wavelength for this assay, but you could read anywhere from 500 to 580 nm, possibly with reduced sensitivity.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
