| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, cell lysate, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of gama-glutamyl transferase enzyme activity in biological samples. Safe and sensitive. Non-radioactive assay. Use as little as 10 µL of sample. Linear detection range in 96-well plate: 0.7 to 200 U/L. The assay uses OD405nm for signal readout. Compatible sample input includes Serum, plasma, cell lysate, and other biological samples. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, cell lysate, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.7 U/L for interpreting low-signal samples.
- Feature emphasis: Safe and sensitive. Non-radioactive assay. Use as little as 10 μL of sample. Linear detection range in 96-well plate: 0.7 to 200 U/L activity.
Additional feature notes highlight Fast and convenient. The procedure involves addition of a single ready-to-use reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of γ-glutamyl transferase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
γ-GLUTAMYL TRANSFERASE(GGT, E.C. 2.3.2.2) is an enzyme that catalyzes the transfer of a glutamyl group to an acceptor, such as a peptide or amino acid. GGT plays a major role in the breakdown of glutathione and is found in many tissues, but is more commonly found in the liver. Elevated levels of GGT may indicate liver disease or damage to the bile ducts, and in clinical practice, it is often tested along with other liver function tests. BioAssay Systems’ DGGT-100 kit provides a convenient colorimetric method to measure GGT activity. In this assay, GGT hydrolyzes a synthetic substrate to release p-nitroanilide (pNA), which absorbs at 405 nm. The increase in absorbance at 405 nm is directly proportional to the enzyme activity.
Detection method
Colorimetric (OD 405 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.7 U/L.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify γ-glutamyl transferase in serum, plasma, cell lysate by OD405 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, cell lysate handling.
- Monitor time-course or pre/post changes in serum, plasma, cell lysate across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this kit come with a positive control?
No, this kit does not include GGT enzyme. This needs to be supplied by the user.
I am encountering high assay background, is this an issue?
There may be increase in background signal of the Reagent over time. However, the calculated results should still be fine since the background is subtracted out when calculating the ΔOD (30 – 0 min).
The results for my samples are not within the reference ranges for GGT.
Please note that the unit definition used for this assay is based on the μmol of pNA generated, which can be different from the units used by clinical reference ranges that are often listed in IU (international units). Reference ranges may also vary depending on the laboratory. Please also keep in mind that this kit is for research use only, and not to be used for diagnostic purposes.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
