| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Compounds that affect glucose-6-phosphate dehydrogenase activity |
| Shipping | |
| Species | |
| Storage |
Overview
For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. The assay uses OD565nm for signal readout. Compatible sample input includes Compounds that affect glucose-6-phosphate dehydrogenase activity. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect glucose-6-phosphate dehydrogenase activity, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 30 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system; Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).
Biological background
This product is centered on measurement of glucose-6-phosphate dehydrogenase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in the survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics. BioAssay System’s non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.
Detection method
Colorimetric (OD 565 nm).
Procedures and timing
Stated procedure or timing information: 30 min.
Screening services
BioAssay Systems offers comprehensive GLucose-6-Phosphate Dehydrogenase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify glucose-6-phosphate dehydrogenase inhibitor screening in compounds that affect glucose-6-phosphate by OD565 nm.
- Compare treatment or phenotype groups using matched compounds that affect glucose-6-phosphate handling.
- Monitor time-course or pre/post changes in compounds that affect glucose-6-phosphate across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with the G6PDH enzymes or control inhibitors?
No, these are not included.
How do I store the kit?
Store all components at -20°C for long term storage.
What species of G6PDH was the kit optimized for?
The kit was optimized for L. Mesenteroides G6PDH.
What if I am interested in testing inhibition of a species besides L. Mesenteroides ?
We recommend that you experimentally determine the Km and then adjust the volume of substrate so that the final concentration of the substrate in the 100 µL Reaction Volume is near the Km.
What G6PDH inhibitor was tested using this kit?
Zn(NO3)2 in dH20.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
