| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food and tissue homogenates |
| Shipping | |
| Species | |
| Storage |
Overview
• For quantitative determination of glycosaminoglycans in food and tissue homogenate. Sensitive and accurate. Use as little as 40 µL samples. Linear detection range in 96-well plate: 0.01 to 0.5 mg/mL hyaluronic acid. Fast and convenient. The assay uses OD600nm for signal readout. Compatible sample input includes Food and tissue homogenates. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD600nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food and tissue homogenates, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.01 mg/mL for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 40 μL samples. Linear detection range in 96-well plate: 0.01 to 0.5 mg/mL hyaluronic acid.
Additional feature notes highlight Fast and convenient. The procedure involves addition of a single working reagent and incubation for 10 min. Room temperature assay. No 37°C incubator is needed; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of glycosaminoglycans within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
GLYCOSAMINOGLYCANS (GAGs)are long, linear polysaccharides and highly negatively charged molecules that constitute a major component of the extracellular matrix in mammalian tissues. GAGs play key roles in cell growth, cell adhesion, wound repair, and anticoagulation. They are also important for brain development and homeostasis. GAGs are associated with osteoarthritis, inflammation, diabetes, spinal cord injury, and cancer.BioAssay Systems’ GAGs assay uses a turbidimetric reaction in which GAGs form an insoluble complex with protein in the reagent. The increase in turbidity at 600nm is directly proportional to the glycosaminoglycans in the sample (reported in hyaluronic acid equivalents).
Detection method
Colorimetric (OD 600 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.01 mg/mL.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify glycosaminoglycans in food and tissue homogenates by OD600 nm readout.
- Compare treatment or phenotype groups using matched food and tissue homogenates handling.
- Monitor time-course or pre/post changes in food and tissue homogenates across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this kit measure all types of GAGs?
All GAGs will be included in the signal; however, it is most accurate for hyaluronic acid. When testing heparin, we found that the signal strength was lower and the shape of the curve was different when compared to a hyaluronic acid standard curve at the same concentrations (mg/mL).
Can this assay be scaled down to 384-well plate format?
We have tested the 384-well format with a similar assay and found that the signal was compromised, most likely because the wells were unable to mix fully.
How do I know if I need a sample blank?
You can compare the absorbance of your sample vs the absorbance of the blank well. In a clear 96-well plate, add 40 μL sample + 160 μL H2O to one well (sample blank) and 40 μL H2O + 160 μL Reagent to another well (blank well). Incubate for 10min at RT then read OD 600nm. If the sample blank is significantly higher than the blank well, then your sample requires a sample blank.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
