QuantiChrom™ Glyoxalase I Assay Kit

Overview

For quantitative determination of glyoxalase enzyme activity. The assay uses OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV) for signal readout. Compatible sample input includes Enzyme preparations or biological samples. Typical stated assay timing is 30 min.

Key elements and design rationale

• Readout format: OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV) supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Enzyme preparations or biological samples, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 4 U/L for interpreting low-signal samples.
• Feature emphasis: Fast and sensitive. Detection limit of 4 U/L GLO-1 activity.

Additional feature notes highlight Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.

Biological background

This product is centered on measurement of glyoxalase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. BioAssay Systems Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.

Detection method

Colorimetric (OD 240 nm).

Detection limit and analytical sensitivity

Reported detection limit: 4 U/L.

Procedures and timing

Stated procedure or timing information: 30 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Quantify glyoxalase in enzyme preparations or biological samples by OD240 nm (Use a UV plate, e.g. 96-well UV plate.
• Compare treatment or phenotype groups using matched enzyme preparations or biological samples handling.
• Monitor time-course or pre/post changes in enzyme preparations or biological samples across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.