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| Sample Type(s) | Blood, serum, plasma, urine etc |
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Overview
For quantitative determination of heme and evaluation of drug effects on heme metabolism. The assay uses OD400nm for signal readout. Compatible sample input includes Blood, serum, plasma, urine etc. Typical stated assay timing is 5 min.
Key elements and design rationale
- Readout format: OD400nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Blood, serum, plasma, urine etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.6 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range 0.6 – 125 µM heme in 96-well plate assay.
Additional feature notes highlight Simple and high-throughput. The “mix-and-read” procedure involves the addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day; Safety. Reagents are non-toxic. Available format information for this listing includes 250 Tests.
Biological background
This product is centered on measurement of heme within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Heme is one important member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalase, and peroxidases. Heme determination is widely practiced by researchers of various blood diseases. Simple, direct, and automation-ready procedures for measuring heme concentration are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ Heme Assay Kit is based on an improved aqueous alkaline solution method, in which the heme is converted into a uniform colored form. The intensity of color, measured at 400 nm, is directly proportional to the heme concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Detection method
Colorimetric (OD 400 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.6 µM.
Procedures and timing
Stated procedure or timing information: 5 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify heme in blood, serum, plasma, urine by OD400 nm readout.
- Compare treatment or phenotype groups using matched blood, serum, plasma, urine handling.
- Monitor time-course or pre/post changes in blood, serum, plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the Heme Assay kit DIHM-250 detect heme bound to hemoglobin or only heme alone?
The heme assay detects the total heme content in the sample, including the heme in hemoglobin.
Does the heme assay detect β-hematin (malaria pigment) / protoporphyrin?
We have not specifically tested it, but it is highly likely that our heme assay will detect β-hematin and protophorphyrin with the same efficiency as heme, and that it is not possible to distinguish between the variants.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Circulating extracellular vesicles from patients with acute chest syndrome disrupt adherens junctions between endothelial cells
Lapping-Carr, G., et al (2020). Circulating extracellular vesicles from patients with acute chest syndrome disrupt adherens junctions between endothelial cells. Pediatric Research. Assay: Heme in human plasma.
SLC46A1 contributes to hepatic iron metabolism by importing heme in hepatocytes
Li, H., et al (2020). SLC46A1 contributes to hepatic iron metabolism by importing heme in hepatocytes. Metabolism, 110, 154306. Assay: Heme in mouse serum.
Heme attenuates endogenous opioid levels in leukocytes of HIV positive individuals with chronic widespread pain
Aggarwal, S., et al (2020). Heme attenuates endogenous opioid levels in leukocytes of HIV positive individuals with chronic widespread pain. Assay: Heme in human and mouse plasma.
Disrupted iron metabolism and mortality during co-infection with malaria and an intestinal Gram-negative Extracellular pathogen
Dos Santos, L. I., et al (2021). Disrupted iron metabolism and mortality during co-infection with malaria and an intestinal Gram-negative Extracellular pathogen. Cell Reports, 34(2), 108613. Assay: Heme in mouse plasma.
Nrf2 activation in myeloid cells and endothelial cells differentially mitigates sickle cell disease pathology in mice
Keleku-Lukwete, N., et al (2019). Nrf2 activation in myeloid cells and endothelial cells differentially mitigates sickle cell disease pathology in mice. Blood Advances, 3(8), 1285-1297. Assay: Heme in mouse plasma.
Heme is involved in the systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas
Yang, X., et al (2020). Heme is involved in the systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas. Assay: Heme in human serum.
Hemopexin as an inhibitor of hemolysis-induced complement activation
Poillerat, V., et al (2020). Hemopexin as an inhibitor of hemolysis-induced complement activation. Frontiers in Immunology, 11. Assay: Heme in mouse plasma.
Heat-killed Fusobacterium nucleatum triggers varying heme-related inflammatory and stress responses depending on primary human respiratory epithelial cell type
Koike, R., et al. (2020). Heat-killed Fusobacterium nucleatum triggers varying heme-related inflammatory and stress responses depending on primary human respiratory epithelial cell type. Molecules, 25(17), 3839. Assay: Heme in cell media.
Endothelial barrier integrity is disrupted in vitro by heme and by serum from sickle cell disease patients
Santaterra, V. A., et al (2020). Endothelial barrier integrity is disrupted in vitro by heme and by serum from sickle cell disease patients. Frontiers in Immunology, 11 Assay: Heme in human serum.
Phosgene Inhalation Causes Hemolysis and Acute Lung Injury
Aggarwal, S., Jilling, T., Doran, S., Ahmad, I., Eagen, J. E., Gu, S. & Patel, R. P. (2019). Phosgene Inhalation Causes Hemolysis and Acute Lung Injury. Toxicology Letters. 521724. Assay: Heme in mice blood.
Nrf2 activation in myeloid cells and endothelial cells differentially mitigates sickle cell disease pathology in mice
Keleku-Lukwete, N., Suzuki, M., Panda, H., Otsuki, A., Katsuoka, F., Saito, R. & Yamamoto, M. (2019). Nrf2 activation in myeloid cells and endothelial cells differentially mitigates sickle cell disease pathology in mice. Blood advances, 3(8), 1285-1297. Assay: Heme in mice plasma.
Metformin affects heme function as a possible mechanism of action
Li, X., Wang, X., & Snyder, M. P. (2019). Metformin affects heme function as a possible mechanism of action. G3: Genes, Genomes, Genetics, 9(2), 513-522. Assay: Heme in yeast cells.
Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction
Belcher, J. D., Chen, C., Nguyen, J., Abdulla, F., Zhang, P., Nguyen, H. & Nath, K. A. (2018). Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction. PloS one, 13(4), e0196455. Assay: Heme in mice plasma.
Distinct inflammatory profile underlies pathological increases in creatinine levels associated with Plasmodium vivax malaria clinical severity
Cruz, L. A., Barral-Netto, M., & Andrade, B. B. (2018). Distinct inflammatory profile underlies pathological increases in creatinine levels associated with Plasmodium vivax malaria clinical severity. PLoS neglected tropical diseases, 12(3), e0006306. Assay: Heme in human blood.
Gingival periodontal disease (PD) level-butyric acid affects the systemic blood and brain organ: insights into the systemic inflammation of periodontal disease
Cueno, M. E., & Ochiai, K. (2018). Gingival periodontal disease (PD) level-butyric acid affects the systemic blood and brain organ: insights into the systemic inflammation of periodontal disease. Frontiers in immunology 9:1158. Assay: Heme in wistar rat blood.
Haemolysis and haem oxygenase-1 induction during persistent “asymptomatic” malaria infection in Burkinabe children
Mooney, J. P., Barry, A., Goncalves, B. P., Tiono, A. B., Awandu, S. S., Grignard, L. & Riley, E. M. (2018). Haemolysis and haem oxygenase-1 induction during persistent “asymptomatic” malaria infection in Burkinabe children. Malaria journal, 17(1), 253. Assay: Heme in human plasma.
Serum haptoglobin and hemopexin levels are depleted in pediatric sickle cell disease patients
Santiago, R. P., Guarda, C. C., Figueiredo, C. V. B., Fiuza, L. M., Aleluia, M. M., Adanho, C. S. A. & Nascimento, V. M. L. (2018). Serum haptoglobin and hemopexin levels are depleted in pediatric sickle cell disease patients. Blood Cells Mol Dis. 72:34-36. Assay: Heme in human blood.
Kinetic Models Demonstrate Ability of Staphylococcus aureus to Uptake Heme from Beta vulgaris Proteins
Abhishek, S., Gupta, A. K., & Singh, A. (2017). Kinetic Models Demonstrate Ability of Staphylococcus aureus to Uptake Heme from Beta vulgaris Proteins. Journal of Pure and Applied Microbiology, 11(4), 1713-1719. Assay: Heme in beetroot juice.
Erythropoietin levels increase during cerebral malaria and correlate with heme, interleukin-10 and tumor necrosis factor-alpha in India
Dalko, E., Tchitchek, N., Pays, L., Herbert, F., Cazenave, P. A., Ravindran, B. & Pied, S. (2016). Erythropoietin levels increase during cerebral malaria and correlate with heme, interleukin-10 and tumor necrosis factor-alpha in India. PloS one, 11(7), e0158420. Assay: Heme in human plasma.
RIPK1 and PGAM5 control Leishmania replication through distinct mechanisms
Luz, N. F., Balaji, S., Okuda, K., Barreto, A. S., Bertin, J., Gough, P. J. & Chan, F. K. M. (2016). RIPK1 and PGAM5 control Leishmania replication through distinct mechanisms. The Journal of Immunology, 196(12), 5056-5063. Assay: Heme in human plasma.
Up-regulating the heme oxygenase system with hemin improves insulin sensitivity and glucose metabolism in adult spontaneously hypertensive rats
Ndisang JF, et al (2010). Up-regulating the heme oxygenase system with hemin improves insulin sensitivity and glucose metabolism in adult spontaneously hypertensive rats. Endocrinology 151(2):549-60. Assay: Heme in rat plasma.
The heme oxygenase system attenuates pancreatic lesions and improves insulin sensitivity and glucose metabolism in deoxycorticosterone acetate hypertension
Ndisang JF, Jadhav A (2010). The heme oxygenase system attenuates pancreatic lesions and improves insulin sensitivity and glucose metabolism in deoxycorticosterone acetate hypertension. American Journal of Physiolgy, Regululatory Integrative and Comparative Physiology. 298(1):R211-23. Assay: Heme in rat tissue.
Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine
Aldag C et al (2009). Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine. PNAS 106(14):5481-6. Assay: Heme in bacteria p450 enzymes.
Elucidation of the mechanism of mitochondrial iron loading in Friedreich’s ataxia by analysis of a mouse mutant
Huang ML et al (2009). Elucidation of the mechanism of mitochondrial iron loading in Friedreich’s ataxia by analysis of a mouse mutant. PNAS 106(38):16381-6. Assay: Heme in mouse heart.
Body iron stores and oxidative damage in humans increased during and after a 10- to 12-day undersea dive
Zwart SR, et al (2009). Body iron stores and oxidative damage in humans increased during and after a 10- to 12-day undersea dive. J Nutr. 139(1):90-5. Assay: Heme in human plasma.
Heme oxygenase-1 and carbon monoxide suppress the pathogenesis of experimental cerebral malaria
Pamplona, A. et al (2007). Heme oxygenase-1 and carbon monoxide suppress the pathogenesis of experimental cerebral malaria. Nature Med. 13(6): 703-710. Assay: Heme in mouse tissue.
