| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | peroxidases and HRP in ELISA |
| Detection limit | |
| Detection method | |
| Plate format | |
| Assay time | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. The assay uses OD450nm for signal readout. Compatible sample input includes peroxidases and HRP in ELISA. Typical stated assay timing is Approx. 30 min.
Key elements and design rationale
- Readout format: OD450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes peroxidases and HRP in ELISA, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 76 nU/well, or 7.7 pg/well for interpreting low-signal samples.
- Feature emphasis: Highly sensitive. Detects as low as 7.6×10 -8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA.
Additional feature notes highlight Improved stability and assay robustness. Available format information for this listing includes 200 Tests.
Biological background
This product is centered on measurement of hrp detection within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
HORSERADISH PEROXIDASE (HRP) is an enzyme commonly used when conjugated to analytes of interest. When combined with hydrogen peroxide and a dye, HRP can be used to colorimetrically quantify the analyst
Detection method
Colorimetric (OD 450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 76 nU/well, or 7.7 pg/well.
Procedures and timing
Stated procedure or timing information: Approx. 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
- Matched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.
Common research applications
- Prepare control-treated samples for paired use with compatible assay-kit plates.
- Benchmark assay response against defined control conditions in replicate wells.
- Optimize reagent concentration and incubation windows for plate-based comparisons.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
