| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Bacterial growth media and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of indole and evaluation of drug effects on its metabolism. The assay uses OD565nm for signal readout. Compatible sample input includes Bacterial growth media and other biological samples. Typical stated assay timing is Under 5 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Bacterial growth media and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 3 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of indole within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
INDOLEis the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole. BioAssay Systems indole assay kit is based on a modified version of Ehlrich’s and Kovac’s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 3 µM.
Procedures and timing
Stated procedure or timing information: Under 5 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify indole in bacterial growth media by OD565 nm readout.
- Compare treatment or phenotype groups using matched bacterial growth media handling.
- Monitor time-course or pre/post changes in bacterial growth media across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the assay work with urine samples
The Assay will detect indole in urine samples; however, the reagent also reacts with urobilinogen in the urine. It does not distinguish between the two.
Does the assay work with serum samples?
The acidity of the reagent often causes serum samples to precipitate which greatly interferes with the assay.
Do I need to read the OD immediately after adding the reagent?
Yes, we recommend you read the OD shortly after adding the reagent to the samples. The OD of the reaction mixture slowly decreases over time. We would recommend reading the OD within 10 minutes of adding the reagent.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
