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| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, saliva, urine, etc |
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Overview
For quantitative determination of lipase enzyme activity. The assay uses OD412nm for signal readout. Compatible sample input includes Serum, plasma, saliva, urine, etc. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD412nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, saliva, urine, etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 40 U/L for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection ranges from 40 to 1600 U/L lipase activity in a 96-well plate assay.
Additional feature notes highlight Convenient and high throughput. The procedure involves adding a single working reagent and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of lipase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
LIPASE catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas. Simple, direct, and automation-ready procedures for measuring lipase activity are very desirable. BioAssay Systems QuantiChrom™ Lipase Assay is based on an improved dimercaptopropanol tributyrate (BALB) method, in which SH groups formed from lipase cleavage of BALB react with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) to form a yellow colored product. The color intensity, measured at 412 nm, is proportionate to the enzyme activity in the sample.
Detection method
Colorimetric (OD 412 nm).
Detection limit and analytical sensitivity
Reported detection limit: 40 U/L.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify lipase in serum, plasma, saliva, urine by OD412 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, saliva, urine handling.
- Monitor time-course or pre/post changes in serum, plasma, saliva, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Can this kit be used to measure lipase activity in cooking oil?
No, we tested it with one oil sample (Sicilian Selezione Extra Virgin Olive Oil). We mixed 600 µL WR with 400 µL oil (control 600 µL WR alone) and shaked for about 4 hrs. We did not see difference in color between control and sample.
Can this kit be used to determine phospholipase activity (phospholipase C)?
Yes, if you use purified phospholipase (e.g.by immunoprecipitation)
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Is frozen human milk that is refused by mother?s own infant suitable for human milk bank donation? Breastfeeding Medicine: The Official Journal of the Academy of Breastfeeding Medicine, 14(4), 271-275
Pitino, MA et al (2019). Is frozen human milk that is refused by mother?s own infant suitable for human milk bank donation? Breastfeeding Medicine: The Official Journal of the Academy of Breastfeeding Medicine, 14(4), 271-275. Assay: Lipase in human milk.
Xbp-1 remodels lipid metabolism to extend longevity
Imanikia, S et al (2019). Xbp-1 remodels lipid metabolism to extend longevity. Cell Reports, 28(3), 581-589.e4. Assay: Lipase in nematode lysates.
Chronic marijuana use moderates the correlations of serum cholesterol with systemic mitochondrial function and fluid cognition
Panee, J et al (2020). Chronic marijuana use moderates the correlations of serum cholesterol with systemic mitochondrial function and fluid cognition. Mitochondrion, 52, 135-143. Assay: Lipase in human serum.
Effects of protocatechuic acid on ameliorating lipid profiles and cardio-protection against coronary artery disease in high fat and fructose diet fed in rats
Li, L et al (2020). Effects of protocatechuic acid on ameliorating lipid profiles and cardio-protection against coronary artery disease in high fat and fructose diet fed in rats. Journal of Veterinary Medical Science, 82(9), 1387-1394. Assay: Lipase in rat serum.
High costs of infection: Alphavirus infection reduces digestive function and bone and feather growth in nestling house sparrows (Passer domesticus)
Fassbinder-Orth, C. A., Killpack, T. L., Goto, D. S., Rainwater, E. L., & Shearn-Bochsler, V. I. (2018). High costs of infection: Alphavirus infection reduces digestive function and bone and feather growth in nestling house sparrows (Passer domesticus). PloS one, 13(4), e0195467. Assay: Lipase in sparrow liver.
PRY-1/Axin signaling regulates lipid metabolism in Caenorhabditis elegans
Ranawade, A., Mallick, A., & Gupta, B. P. (2018). PRY-1/Axin signaling regulates lipid metabolism in Caenorhabditis elegans. PloS one, 13(11), e0206540. Assay: Lipase in worm tissue.
The Proteomic Analysis of Pancreatic Exocrine Insufficiency Protein Marker in Type 2 Diabetes Mellitus Patients
Srihardyastutie, A., & Soeatmadji, D. W. (2018). The Proteomic Analysis of Pancreatic Exocrine Insufficiency Protein Marker in Type 2 Diabetes Mellitus Patients. In IOP Conference Series: Materials Science and Engineering 299(1) p 012021. Assay: Lipase in human blood.
Pasteurization procedures for donor human milk affect body growth, intestinal structure, and resistance against bacterial infections in preterm pigs
Li, Y., Nguyen, D. N., de Waard, M., Christensen, L., Zhou, P., Jiang, P. & Dalsgaard, T. K. (2017). Pasteurization procedures for donor human milk affect body growth, intestinal structure, and resistance against bacterial infections in preterm pigs. The Journal of nutrition, 147(6), 1121-1130. Assay: Lipase in human milk.
NAD+ augmentation ameliorates acute pancreatitis through regulation of inflammasome signalling
Shen, A., Kim, H. J., Oh, G. S., Lee, S. B., Lee, S. H., Pandit, A. & Cho, E. Y. (2017). NAD+ augmentation ameliorates acute pancreatitis through regulation of inflammasome signalling. Scientific reports, 7(1), 3006. Assay: Lipase in mice serum.
Glycerol monolaurate inhibits lipase production by clinical ocular isolates without affecting bacterial cell viability
Flanagan, J. L., Khandekar, N., Zhu, H., Watanabe, K., Markoulli, M., Flanagan, J. T., & Papas, E. (2016). Glycerol monolaurate inhibits lipase production by clinical ocular isolates without affecting bacterial cell viability. Investigative ophthalmology & visual science, 57(2), 544-550. Assay: Lipase in bacteria cells.
The effect of storage at 25 deg C on proteins in human milk
Molinari, C.E., et al. (2011). The effect of storage at 25 deg C on proteins in human milk. International Dairy Journal 21(4):286-293. Assay: Lipase in human milk.
Effect of bile on the oral absorption of halofantrine in polyethylene glycol 400 and polysorbate 80 formulations dosed to bile duct cannulated rats
Tonsberg, H., et al. (2011). Effect of bile on the oral absorption of halofantrine in polyethylene glycol 400 and polysorbate 80 formulations dosed to bile duct cannulated rats. J Pharm Pharmacol 63(6):817-24. Assay: Lipase in rat intestine.
human risk allele HLA-DRB1*0405 predisposes class II transgenic Ab0 NOD mice to autoimmune pancreatitis
Freitag, T.L., et al. (2010). human risk allele HLA-DRB1*0405 predisposes class II transgenic Ab0 NOD mice to autoimmune pancreatitis. Gastroenterology 139(1):281-91. Assay: Lipase in mouse serum.
Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura)
Hu, M., et al. (2010). Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). Journal of Experimental Marine Biology and Ecology 395(1-2):112-9. Assay: Lipase in horseshoe crab blood.
Biochemical and histological effects of exendin-4 (exenatide) on the rat pancreas
Nachnani, J.S., et al. (2010). Biochemical and histological effects of exendin-4 (exenatide) on the rat pancreas. Diabetologia 53(1):153-9. Assay: Lipase in rat serum.
Methods for detection and characterization of lipases: A comprehensive review
Hasan, F., et al. (2009). Methods for detection and characterization of lipases: A comprehensive review. Biotechnol Adv 27(6):782-98. Assay: Lipase in microbes.
Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway
Mudgil, Y., et al. (2009). Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway. Plant Cell 21(11):3591-609. Assay: Lipase in Arabidopsis thaliana recombinant proteins.
Caenorhabditis elegans dauers need LKB1/AMPK to ration lipid reserves and ensure long-term survival
Narbonne, P., Roy, R. (2009). Caenorhabditis elegans dauers need LKB1/AMPK to ration lipid reserves and ensure long-term survival. Nature 457(7226):210-4. Assay: Lipase in C.elegans larvae.
Macrophage-derived human resistin exacerbates adipose tissue inflammation and insulin resistance in mice
Qatanani, M., et al. (2009). Macrophage-derived human resistin exacerbates adipose tissue inflammation and insulin resistance in mice. J Clin Invest 119(3):531-9. Assay: Lipase in mouse muscle tissue.
Pancreatic exocrine insufficiency in LXRbeta-/- mice is associated with a reduction in aquaporin-1 expression
Gabbi, C., et al. (2008). Pancreatic exocrine insufficiency in LXRbeta-/- mice is associated with a reduction in aquaporin-1 expression. Proc Natl Acad Sci U S A 105(39):15052-7. Assay: Lipase in mouse serum.
