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| Sample Type(s) | Plasma, serum, urine, tissue/cells, and foods |
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Overview
For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. The assay uses OD540nm for signal readout. Compatible sample input includes Plasma, serum, urine, tissue/cells, and foods. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: OD540nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Plasma, serum, urine, tissue/cells, and foods, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.6 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Detection range 0.6 – 100 µM in a 96-well plate.
Additional feature notes highlight Rapid and reliable. Using an optimized VCl 3 reagent, the time required for the reduction of nitrate (NO 3 – ) to nitrite (NO 2 – ) is reduced to 10 min at 60°C; Simple and high-throughput. The procedure involves mixing the sample with three reagents, incubation for 10 min at 60°C, and reading the optical density. Can be readily automated to measure thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of nitric oxide within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Simple, direct, and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3-as a measure of NO level. BioAssay Systems QuantiChrom™ Nitric Oxide Assay Kit is designed to accurately measure NO production following the reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 min.
Detection method
Colorimetric (OD 540 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.6 µM.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify nitric oxide in plasma, serum, urine by OD540 nm readout.
- Compare treatment or phenotype groups using matched plasma, serum, urine handling.
- Monitor time-course or pre/post changes in plasma, serum, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Non-neuronal cardiac acetylcholine system playing indispensable roles in cardiac homeostasis confers resiliency to the heart
Oikawa, S., et al. (2021). Non-neuronal cardiac acetylcholine system playing indispensable roles in cardiac homeostasis confers resiliency to the heart. The Journal of Physiological Sciences 71(1). Assay: Nitric Oxide in mouse cardiac tissue.
A strategy for component-based Chinese medicines design approach of Polygonum orientale L
Wan, S., et al. (2021). A strategy for component-based Chinese medicines design approach of Polygonum orientale L. against hypoxia/reoxygenation based on uniform design-stepwise regression-simulated annealing. Biomedicine & Pharmacotherapy 135. Assay: Nitric Oxide in polygonum orientale L cells.
Sulforaphane prevents type 2 diabetes-induced nephropathy via AMPK-mediated activation of lipid metabolic pathways and Nrf2 antioxidative function
Li, Z., et al. (2020). Sulforaphane prevents type 2 diabetes-induced nephropathy via AMPK-mediated activation of lipid metabolic pathways and Nrf2 antioxidative function. Clinical Science 134(18): 2469-2487. Assay: Nitric Oxide in mouse urine.
Analysis of haemodynamics and angiogenic response to ischaemia in the obese type 2 diabetic model Spontaneously Diabetic Torii Leprfa (SDT fatty) rats
Murai, Y., et al. (2020). Analysis of haemodynamics and angiogenic response to ischaemia in the obese type 2 diabetic model Spontaneously Diabetic Torii Leprfa (SDT fatty) rats. Clinical and Experimental Pharmacology & Physiology 47(4): 583-590. Assay: Nitric Oxide in rat plasma.
Analysis of the effects of Cu-MOFs on fungal cell inactivation
Veerana, M., et al. (2021). Analysis of the effects of Cu-MOFs on fungal cell inactivation. RSC Advances 11(2): 1057-1065. Assay: Nitric Oxide in fungi cells and spores.
SARS-CoV-2 induces mitochondrial dysfunction and cell death by oxidative stress in leukocytes of COVID-19 patients
De la Cruz-Enriquez, J., et al. (2020). SARS-CoV-2 induces mitochondrial dysfunction and cell death by oxidative stress in leukocytes of COVID-19 patients. Authorea. Assay: Nitric Oxide in human plasma.
Pretreatment with cilnidipine attenuates hypoxia/reoxygenation injury in HL-1 cardiomyocytes through enhanced NO production and action potential shortening
Minato, H., et al. (2020). Pretreatment with cilnidipine attenuates hypoxia/reoxygenation injury in HL-1 cardiomyocytes through enhanced NO production and action potential shortening. Hypertension Research 43(5): 380-388. Assay: Nitric Oxide in mouse cardiomyocytes.
Plasma-treated Flammulina velutipes-derived extract showed anticancer potential in human breast cancer cells
Mitra, S., et al. (2020). Plasma-treated Flammulina velutipes-derived extract showed anticancer potential in human breast cancer cells. Applied Sciences 10(23). Assay: Nitric Oxide in gas plasma.
Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
Fischer, A., et al. (2020). Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity. Journal of Clinical Biochemistry and Nutrition 66(1): 24-35. Assay: Nitric Oxide in mouse blood.
Resveratrol inhibits neointimal growth after arterial injury in high-fat-fed rodents: The roles of sirt1 and ampk
Guo, J., et al. (2020). Resveratrol inhibits neointimal growth after arterial injury in high-fat-fed rodents: The roles of sirt1 and ampk. Journal of Vascular Research 57(6): 325-340. Assay: Nitric Oxide in rat plasma.
Plasma mediated disinfection of rice seeds in water and air
Kang, M., et al. (2020). Plasma mediated disinfection of rice seeds in water and air. Journal of Physics D: Applied Physics 53(21). Assay: Nitric Oxide in .
A mechanistic and kinetic study of diazinone degradation under the influence of microplasma discharge water
Khan, M. S. I., et al. (2020). A mechanistic and kinetic study of diazinone degradation under the influence of microplasma discharge water. Journal of Water Process Engineering 36. Assay: Nitric Oxide in discharge water.
Supplementation of 100% flesh watermelon [Citrullus lanatus (Thunb
Ridwan, R., et al. (2019). Supplementation of 100% flesh watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] juice improves swimming performance in rats. Preventive Nutrition and Food Science 24(1): 41-48. Assay: Nitric Oxide in rat plasma.
Plasma-mediated enhancement of enzyme secretion in Aspergillus oryzae
Veerana, M., et al. (2021). Plasma-mediated enhancement of enzyme secretion in Aspergillus oryzae. Microbial Biotechnology 14(1): 262-276. Assay: Nitric Oxide in fungal media.
Influence of non-thermal atmospheric pressure plasma jet on extracellular activity of α-amylase in Aspergillus oryzae
Veerana, M., et al. (2021). Influence of non-thermal atmospheric pressure plasma jet on extracellular activity of α-amylase in Aspergillus oryzae. Applied Sciences 11(2). Assay: Nitric Oxide in PDB media.
Cobalt(Ii)-coordination polymers containing glutarates and bipyridyl ligands and their antifungal potential
Kim, H.-C., et al. (2019). Cobalt(Ii)-coordination polymers containing glutarates and bipyridyl ligands and their antifungal potential. Scientific Reports 9(1). Assay: Nitric Oxide in cobalt(II)-coordination polymer solution.
Vitamin D influences calcium dependent cardiovascular functions with reference to NOS3 and VEGF
Lata, M.-M. (2019). Vitamin D influences calcium dependent cardiovascular functions with reference to NOS3 and VEGF. BLDE. Assay: Nitric Oxide in human serum.
Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster
Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Nitric oxidein plant tissues.
gamma-Secretase component presenilin is important for microglia beta-amyloid clearance
Farfara D, et al (2011). gamma-Secretase component presenilin is important for microglia beta-amyloid clearance. Ann Neurol. 69(1):170-80. Assay: Nitric oxidein mouse tissue culture.
Assay Of Nitric Oxide Metabolites In The Culture Media Of human Preimplantation Embryosus
Lipari et al (2011). Assay Of Nitric Oxide Metabolites In The Culture Media Of human Preimplantation Embryosus. US 2011/0009689 A1. Assay: Nitric Oxide in human tissue culture.
Control of Cervicovaginal HPV-16 E7-Expressing Tumors by the Combination of Therapeutic HPV Vaccination and Vascular Disrupting Agents
Zeng Q, et al (2011). Control of Cervicovaginal HPV-16 E7-Expressing Tumors by the Combination of Therapeutic HPV Vaccination and Vascular Disrupting Agents. Hum Gene Ther. 22(7):809-19. Assay: Nitric oxidein human tissue.
Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta
Alonso F, et al (2010). Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta. Am J Physiol Heart Circ Physiol. 299(5):H1365-73. Assay: Nitric oxidein mouse aorta.
N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats
Jawed H, et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol. 10(8):900-5. Assay: Nitric oxidein rat serum and plasma.
The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma
Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Nitric Oxide in human blood.
Increment of interleukin 6, tumour necrosis factor alpha, nitric oxide, C-reactive protein and apoptosis in dengue
Levy A, et al (2010). Increment of interleukin 6, tumour necrosis factor alpha, nitric oxide, C-reactive protein and apoptosis in dengue. Trans R Soc Trop Med Hyg. 104(1):16-23. Assay: Nitric oxidein human fluid, tissue and cells.
Characterization of brain lesions in a mouse model of progressive multiple sclerosis
Levy H, et al (2010). Characterization of brain lesions in a mouse model of progressive multiple sclerosis. Exp Neurol. 226(1):148-58. Assay: Nitric oxidein mouse tissue.
Modulation of murine innate and acquired immune responses following in vitro exposure to electrospun blends of collagen and polydioxanone
Smith MJ, et al (2010). Modulation of murine innate and acquired immune responses following in vitro exposure to electrospun blends of collagen and polydioxanone. J Biomed Mater Res A. 93(2):793-806. Assay: Nitric oxidein mouse tissue.
Increased vascular endothelial growth factor expression, CD3-positive cell infiltration, and oxidative stress in premalignant lesions of the cervix
Carrero Y, et al (2009). Increased vascular endothelial growth factor expression, CD3-positive cell infiltration, and oxidative stress in premalignant lesions of the cervix. Cancer 115(16):3680-8. Assay: Nitric oxidein human cervix and serum.
Ranolazine improves endothelial function in patients with stable coronary artery disease
Deshmukh SH, et al (2009). Ranolazine improves endothelial function in patients with stable coronary artery disease. Coron Artery Dis. 20(5):343-7. Assay: Nitric oxidein human tissue.
Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation
Lipari CW, et al (2009). Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation. Fertil Steril. 91(4 Suppl):1316-8. Assay: Nitric oxidein human tissue culture.
Elevated level of endothelin-1 in cerebrospinal fluid and lack of nitric oxide in basilar arterial plasma associated with cerebral vasospasm after subarachnoid haemorrhage in rabbits
Neuschmelting V, et al (2009). Elevated level of endothelin-1 in cerebrospinal fluid and lack of nitric oxide in basilar arterial plasma associated with cerebral vasospasm after subarachnoid haemorrhage in rabbits. Acta Neurochir (Wien) 151(7):795-801. Assay: Nitric oxidein rabbit cerebral arterial plasma and cerebrospinal fluid.
Endogenous n-3 fatty acids protect ovariectomy induced bone loss by attenuating osteoclastogenesis
Rahman MM, et al (2009). Endogenous n-3 fatty acids protect ovariectomy induced bone loss by attenuating osteoclastogenesis. J Cell Mol Med. 13(8B):1833-44. Assay: Nitric oxidein mouse bone marrow.
In vitro evaluations of innate and acquired immune responses to electrospun polydioxanone-elastin blends
Smith MJ, et al (2009). In vitro evaluations of innate and acquired immune responses to electrospun polydioxanone-elastin blends. Biomaterials 30(2):149-59. Assay: Nitric oxidein mouse cells.
Pioglitazone increases adiponectin levels in nondiabetic patients with coronary artery disease
Patel SR, et al (2008). Pioglitazone increases adiponectin levels in nondiabetic patients with coronary artery disease. Coron Artery Dis.19(5):349-53. Assay: Nitric oxidein human tissue.
Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia
Wang SH,et al (2008). Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia. Microbes Infect. 10(4):334-41. Assay: Nitric oxidein mouse BALF.
Dietary salt activates an endothelial proline-rich tyrosine kinase 2/c-Src/phosphatidylinositol 3-kinase complex to promote endothelial nitric oxide synthase phosphorylation
Ying WZ, et al (2008). Dietary salt activates an endothelial proline-rich tyrosine kinase 2/c-Src/phosphatidylinositol 3-kinase complex to promote endothelial nitric oxide synthase phosphorylation. Hypertension 52(6):1134-41. Assay: Nitric oxidein rat urine.
Analysis of methylarginine metabolism in the cardiovascular system identifies the lung as a major source of ADMA
Bulau P, et al (2007). Analysis of methylarginine metabolism in the cardiovascular system identifies the lung as a major source of ADMA. Am J Physiol Lung Cell MolPhysiol. 292(1):L18-24. Assay: Nitric oxidein mouse tissue.
Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2
Hasegawa K, et al (2007). Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2. Circ Res. 101(2):e2-10. Assay: Nitric oxidein mouse tissue extracts.
The compartmentalization of prolactin signaling in the mouse mammary gland
Bolander FF. (2005). The compartmentalization of prolactin signaling in the mouse mammary gland. Mol Cell Endocrinol. 245(1-2):105-10. Assay: Nitric oxidein mouse mammary cells.
