| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Enzyme extracts, agriculture, and biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of pectinase activity determination in biological samples. The assay uses OD600nm for signal readout. Compatible sample input includes Enzyme extracts, agriculture, and biological samples. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: OD600nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Enzyme extracts, agriculture, and biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 9.2 U/L for interpreting low-signal samples.
- Feature emphasis: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay.
Additional feature notes highlight Simple and convenient 40-minute “Add-Mix-Measure” type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating is required; The kit does not use toxic materials. Refer to the previously established DNS method. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of pectinase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PECTINASE (EC 3.2.1.15)is an enzyme that catalyzes the hydrolysis of pectin, a heteropolysaccharide found primarily in the middle lamella and cell wall of terrestrial plants. During fruit ripening, enzymes, including pectinase break down the middle lamella, separating cells and softening the fruit. Pectinase enzymes are used in the food industry to liquefy fruit pulp and clarify fruit juice and wine.BioAssay Systems’ DPEC-100 kit provides a rapid and convenient assay for pectinase enzyme activity, in which pectin the substrate forms a turbid complex with the detection reagent. The turbidity, measured at OD600nm, is proportional to the amount of unhydrolyzed pectin, thus inversely proportional to the pectinase activity.
Detection method
Colorimetric (OD 600 nm).
Detection limit and analytical sensitivity
Reported detection limit: 9.2 U/L.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify pectinase in enzyme extracts, agriculture, and biological by OD600 nm readout.
- Compare treatment or phenotype groups using matched enzyme extracts, agriculture, and biological handling.
- Monitor time-course or pre/post changes in enzyme extracts, agriculture, and biological across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Will pectin lyase work with this kit?
Pectin lyase (EC 4.2.2.10) and other enzymes that hydrolyze pectin will likely work with this kit, however the enzyme unit definition for this kit refers to endopolygalacturonase (EC 3.2.1.15). So the results of this kit are reported as activity of EC 3.2.1.15.
Why is there no pectinase enzyme standard in this kit?
Pectinase may not remain stable so pectinase activity is matched with the included calibrator. The optical density of the calibrator matches the optical density of wells assayed with 100 U/L pectinase under the standard assay conditions.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
