| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of plasmin enzyme activity in biological samples. The assay uses OD405nm for signal readout. Compatible sample input includes Serum and other biological samples. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.35 U/L for interpreting low-signal samples.
- Feature emphasis: Safe and sensitive. Non-radioactive assay. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.35 to 56.5 U/L activity.
Additional feature notes highlight Fast and convenient. The procedure involves addition of a single working reagent and incubation for 30 min; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of plasmin within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PLASMIN(3.4.21.7) is a serine protease that is a key enzyme in the process of fibrinolysis. Plasmin is released into circulation as its inactive form, plasminogen, which can be activated by enzymes such as urokinase. The plasmin/plasminogen system has been studied for its role in inflammation, degradation of the extracellular matrix, and wound healing. BioAssay Systems’ DPLM-100 kit provides a convenient colorimetric method to measure plasmin activity. In this assay, plasmin hydrolyzes a synthetic substrate to release p-nitroanilide (pNA), which absorbs at 405 nm. The increase in absorbance at 405 nm is directly proportional to the enzyme activity.
Detection method
Colorimetric (OD 405 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.35 U/L.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify plasmin in serum by OD405 nm readout.
- Compare treatment or phenotype groups using matched serum handling.
- Monitor time-course or pre/post changes in serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this kit come with plasmin?
No, this kit does not come with a plasmin positive control.
Why is the signal from my samples is very low?
It is possible that much of the plasmin is present in the form of plasminogen, which is inactive and will not react with the substrate in the kit. Plasminogen needs to be activated, for example by incubation with streptokinase or urokinase.
My kit was delayed during shipping, will it still work?
This kit is stable for 10 days at room temperature, so the kit is most likely fine.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
