QuantiChrom™ Protein Assay Kit

Overview

For quantitative determination of total protein. The assay uses OD595nm (Bradford) for signal readout. Compatible sample input includes Biological, food, agriculture etc. Typical stated assay timing is 2 min.

Key elements and design rationale

• Readout format: OD595nm (Bradford) supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Biological, food, agriculture etc, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 60 µg/mL for interpreting low-signal samples.
• Feature emphasis: Sensitive and accurate. Use 10 mL samples. Detection range 0.06 – 1.0 mg /mL protein in 96-well plate assay.

Additional feature notes highlight Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day; Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay. Available format information for this listing includes 500 Tests.

Biological background

This product is centered on measurement of protein within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

The protein is known as the “building blocks of life” and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. BioAssay Systems QuantiChrom™ protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.

Detection method

Colorimetric (OD 595 nm).

Detection limit and analytical sensitivity

Reported detection limit: 60 µg/mL.

Procedures and timing

Stated procedure or timing information: 2 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Quantify protein in biological, food, agriculture by OD595 nm (Bradford) readout.
• Compare treatment or phenotype groups using matched biological, food, agriculture handling.
• Monitor time-course or pre/post changes in biological, food, agriculture across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.