| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Urine samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). The assay uses OD600 and OD530nm for signal readout. Compatible sample input includes Urine samples. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD600 and OD530nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Urine samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1 mg/dL Protein and 1 mg/dL Creatinine for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 – 20 mg/dL Protein and 1 – 150 mg/dL Creatinine.
Additional feature notes highlight Fast and convenient. No sample pre-treatment is needed. Simple 10- minute “add-incubate-read” procedure; High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of protein creatinine ratio within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PROTEINis filtered out of urine by the glomeruli of the kidneys. Albumin is the most common serum protein, thus the majority of the protein in urine is albumin. A damaged kidney will allow some protein through into the urine, the less protein in urine the better. Elevated protein levels in urine are called microalbuminuria or proteinuria, which typically arises due to type 1 diabetes, type 2 diabetes, or high blood pressure. CREATININE is synthesized in the body at a fairly constant rate from creatine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. PROTEIN/CREATININE RATIO (PCR) remains the simplest and most convenient test for proteinuria. Other methods such as 24-hour urine tests or timed urine tests require strict adherence to sample collection protocol. Since the protein concentration is normalized to creatinine secretion, the urine sample can be taken at any time and no diet or liquid restrictions are necessary for sample collection.
Detection method
Colorimetric (OD 600 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify protein creatinine ratio in urine samples by OD600 and OD530 nm readout.
- Compare treatment or phenotype groups using matched urine samples handling.
- Monitor time-course or pre/post changes in urine samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the assay kit work in a particular species?
Yes, this kit works for urine of all species.
Do I need to run an internal standard for each sample?
Yes, urine varies widely from individual to individual. Depending on the concentration of the urine and the diet of the individual, each sample will respond to the reagent slightly differently. The internal standard corrects for the slight differences thus calculating accurate results.
Do I need to run a blank for each sample?
No, you only need to run a blank in duplicate for each assay. For example, if you are running 5 samples, you will have 10 internal standard wells, 10 sample wells, and 2 blank wells.
Can I store unused reagents for future use?
Yes, unused reagents can be stored in the refridgerator until next use. Be sure to equilibrate reagents to room temperature before using again.
Do I need to use a standard curve? Why is there no standard curve in the protocol?
For assays that use an internal standard, running a standard for each sample would be cumbersome, and would consume large quantities of reagents. It is standard practice to use a one point standard to calculate your values.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
3′-O-beta-d-glucopyranosyl-alpha, 4, 2′, 4′, 6′-pentahydroxy-dihydrochalcone, from Bark of Eysenhardtia polystachya Prevents Diabetic Nephropathy via Inhibiting Protein Glycation in STZ-Nicotinamide Induced Diabetic Mice
Perez Gutierrez, R. M., Garcia Campoy, A. H., Paredes Carrera, S. P., Muniz Ramirez, A., Mota Flores, J. M., Valle, F., & Odin, S. (2019). 3′-O-beta-d-glucopyranosyl-alpha, 4, 2′, 4′, 6′-pentahydroxy-dihydrochalcone, from Bark of Eysenhardtia polystachya Prevents Diabetic Nephropathy via Inhibiting Protein Glycation in STZ-Nicotinamide Induced Diabetic Mice. Molecules, 24(7), 1214. Assay: Protein creatinine ratio in mice serum.
Renal dysfunction aggravated impaired cutaneous wound healing in diabetic mice
Xie, P., Young, M. W., Bian, H., Niknam-Bienia, S., Hong, S., Mustoe, T. A., & Galiano, R. D. (2019). Renal dysfunction aggravated impaired cutaneous wound healing in diabetic mice. Wound Repair and Regeneration, 27(1), 49-58. Assay: Protein creatinine ratio in mice blood.
