| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For detection and quantification of pyrophosphatase enzyme activity. The assay uses OD620 for signal readout. Compatible sample input includes Biological samples. Typical stated assay timing is 1 hr.
Key elements and design rationale
- Readout format: OD620 supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1.0 U/L for interpreting low-signal samples.
- Feature emphasis: Safe and sensitive. Non-radioactive assay. Use as little as 10 μL samples. Linear detection range in 96-well plate: 1.0 to 20 U/L activity.
Additional feature notes highlight Fast and convenient. The procedure involves addition of a single working reagent and incubation for 60 min. Room temperature assay. No 37°C incubator is needed; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of pyrophosphatase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
INORGANIC PYROPHOSPHATASE(E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds on inorganic pyrophosphate [P2O74-], thereby releasing two orthophosphate molecules. Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid β-oxidation. Simple, direct and automation-ready procedures for measuring pyrophosphatase activity are very desirable. BioAssay Systems’ DPPT-100 assay is based on our proprietary phosphate assay kit (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from pyrophosphate hydrolysis.
Detection method
Colorimetric (OD 620 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1.0 U/L.
Procedures and timing
Stated procedure or timing information: 1 hr.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify pyrophosphatase in biological samples by OD620 readout.
- Compare treatment or phenotype groups using matched biological samples handling.
- Monitor time-course or pre/post changes in biological samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Will this kit work on Family I/II/M PPases?
This kit was developed using a family I Ppase, and we cannot guarantee reliable results for a family II or membrane PPases.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
