| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For assessing PDH activity in biological samples and screening and evaluation of PDH modulators. High sensitivity and wide linear range. The detection limit is 0.87 U/L, with linearity up to at least 278 U/L PDH in a 96-well plate assay. The assay uses OD565nm for signal readout. Compatible sample input includes Biological samples. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.87 U/L for interpreting low-signal samples.
- Feature emphasis: High sensitivity and wide linear range. The detection limit is 0.87 U/L, with linearity up to at least 278 U/L PDH in a 96-well plate assay.
Additional feature notes highlight Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of PDH activity within 20 minutes; Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of pyruvate dehydrogenase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PYRUVATE DEHYDROGENASE (PDH) (E.C. 1.2.4.1) is an oxidoreductase which catalyzes the decarboxylation of pyruvate to form acetylated dihydrolipoamide in a thiamine pyrophosphate (TPP)-dependent reaction. PDH is the E1 component of the PDH complex that ultimately produces acetyl-CoA. The PDH complex connects Glycolysis to the Citric Acid Cycle. This non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt MTT in a Phenazine Methosulfate (PMS) coupled reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the PDH activity.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.87 U/L.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify pyruvate dehydrogenase in biological samples by OD565 nm readout.
- Compare treatment or phenotype groups using matched biological samples handling.
- Monitor time-course or pre/post changes in biological samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit include Pyruvate Dehydrogenase enzyme?
No, the researcher must provide their own Pyruvate Dehydrogenase enzyme, but the kit was developed using purified PDH from porcine heart muscle (Sigma P-7032).
Does this kit measure the PDH complex activity?
This kit is used to measure the enzyme activity of the E1 subunit of the PDH complex, so neither NAD nor FAD are required for the assay.
Can I let the plate sit out between the T0 minute read and the T20 minute read?
The plate needs to be protected from the light during its entire incubation as the developing reagent (PMS-MTT) is very photosensitive.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
