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| Sample type(s) | Serum, plasma, urine, saliva etc |
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Overview
For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. The assay uses OD535nm, or FL530/550nm for signal readout. Compatible sample input includes Serum, plasma, urine, saliva etc. Typical stated assay timing is 80 min.
Key elements and design rationale
- Readout format: OD535nm, or FL530/550nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, saliva etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.1 µM for interpreting low-signal samples.
Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of tbars within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. BioAssay Systems TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 530nm/550nm) is directly proportional to TBARS concentration in the sample.
Detection method
Colorimetric (OD 535 nm) or Fluorescent (FL 530/550 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.1 µM.
Procedures and timing
Stated procedure or timing information: 80 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify tbars in serum, plasma, urine, saliva by OD535 nm, or FL530/550 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine, saliva handling.
- Monitor time-course or pre/post changes in serum, plasma, urine, saliva across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What is the principle of the TBARS assay?
Biological specimens contain a mixture of thiobarbituric acid reactive substances (TBARS), including lipid hydroperoxides and aldehydes, which increase as a result of oxidative stress. Malondialdehyde (MDA) forms a 1:2 adduct with thiobarbituric acid. TBARS are expressed in terms of malondialdehyde (MDA) equivalents.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
D4F prophylaxis enables redox and energy homeostasis while preventing inflammation during hypoxia exposure
Paul, S., et al. (2021). D4F prophylaxis enables redox and energy homeostasis while preventing inflammation during hypoxia exposure. Biomedicine & Pharmacotherapy. 133: 111083. Assay: TBARS in rat plasma.
Resveratrol and quercetin attenuate depressive-like behavior and restore impaired contractility of vas deferens in chronic stress-exposed rats: Involvement of oxidative stress and inflammation
Sahin, T. D. et al. (2020). Resveratrol and quercetin attenuate depressive-like behavior and restore impaired contractility of vas deferens in chronic stress-exposed rats: Involvement of oxidative stress and inflammation. Naunyn-Schmiedeberg ‘s Archives of Pharmacology. 393(5): 761-775. Assay: TBARS in rat vas deferens tissue.
Effect of dietary curcumin on the antioxidant status of laying hens under high- temperature condition
Nawab, A., et al. (2019). Effect of dietary curcumin on the antioxidant status of laying hens under high- temperature condition. Journal of Thermal Biology. 86: 102449. Assay: TBARS in hen serum.
Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization
Jain, S., et al. (2020). Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization. Nitric Oxide. 95: 1-11. Assay: TBARS in human saliva.
Quantitative proteomics reveal an altered pattern of protein expression in saliva of hypobaric hypoxia-induced rat model
Jain, S., et al. (2020). Quantitative proteomics reveal an altered pattern of protein expression in saliva of hypobaric hypoxia-induced rat model. Journal of Proteins and Proteomics. 11(2): 81-92. Assay: TBARS in rat saliva.
Ganoderma lucidum polysaccharide peptide reduce inflammation and oxidative stress in patient with atrial fibrillation
Rizal, A.,et al. (2020). Ganoderma lucidum polysaccharide peptide reduce inflammation and oxidative stress in patient with atrial fibrillation. The Indonesian Biomedical Journal. 12(4): 384-389. Assay: TBARS in human plasma.
Silencing of long non-coding antisense RNA brain-derived neurotrophic factor attenuates hypoxia/ischemia-induced neonatal brain injury
Qiao, L., et al. (2020). Silencing of long non-coding antisense RNA brain-derived neurotrophic factor attenuates hypoxia/ischemia-induced neonatal brain injury. International Journal of Molecular Medicine. 46(2): 653-662. Assay: TBARS in rat neural tissue cells.
Effect of storage on n-3 PUFA-enriched eggs
Liang, K., et al. (2020). Effect of storage on n-3 PUFA-enriched eggs. CyTA – Journal of Food. 18(1): 102-107. Assay: TBARS in chicken eggs.
Glucocorticoid exposure induces preeclampsia via dampeninglipoxin a4, an endogenous anti-inflammatory and proresolving mediator
Liu, H., et al. (2020). Glucocorticoid exposure induces preeclampsia via dampeninglipoxin a4, an endogenous anti-inflammatory and proresolving mediator. Frontiers in Pharmacology. 11: 1131. Assay: TBARS in rat placenta.
Flavonoids regulate cell death-related cellular signaling via ROS in human colon cancer cells
Dukel, M., et al. (2021). Flavonoids regulate cell death-related cellular signaling via ROS in human colon cancer cells. Process Biochemistry. 101: 11-25. Assay: TBARS in human colorectal cells.
Chlorogenic acid improves the quality of boar semen processed in Percoll
Rabelo, S. S., et al. (2020). Chlorogenic acid improves the quality of boar semen processed in Percoll. Animal Reproduction. 17(1): e20190021. Assay: TBARS in boar seminal plasma.
Lipoxin A4 suppresses angiotensin II type 1 receptor autoantibody in preeclampsia via modulating caspase-1
Liu, H., et al. (2020). Lipoxin A4 suppresses angiotensin II type 1 receptor autoantibody in preeclampsia via modulating caspase-1. Cell Death & Disease. 11(1): 78. Assay: TBARS in mouse placenta.
Induction of nitric oxide synthesis: A strategy to defend against high environmental ammonia-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur
Hasan, R., et al. (2020). Induction of nitric oxide synthesis: A strategy to defend against high environmental ammonia-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur. The Journal of Experimental Biology. 223(9). Assay: TBARS in catfish hepatocytes.
Whole blood haemostatic function throughout a 28-day cold storage period: An in vitro study
McRae, H. L., et al. (2020). Whole blood haemostatic function throughout a 28-day cold storage period: An in vitro study. Vox Sanguinis. Assay: Hemoglobin in human blood.
The hidden role of paxillin: Localization to nucleus promotes tumor angiogenesis
Noh, K., et al (2020). The hidden role of paxillin: Localization to nucleus promotes tumor angiogenesis. Oncogene, 40(2), 384-395. Assay: Hemoglobin in mouse matrigel plug.
Overexpression of FAM46A, a non-canonical Poly(A) polymerase, promotes hemin-induced Hemoglobinization in K562 cells
Lin, H., et al. (2020). Overexpression of FAM46A, a non-canonical Poly(A) polymerase, promotes hemin-induced Hemoglobinization in K562 cells. Frontiers in Cell and Developmental Biology, 8. Assay: Hemoglobin in mouse cells.
Exosomal delivery of AntagomiRs targeting viral and cellular MicroRNAs synergistically inhibits cancer angiogenesis
Wang, J., et al (2020). Exosomal delivery of AntagomiRs targeting viral and cellular MicroRNAs synergistically inhibits cancer angiogenesis. Molecular Therapy – Nucleic Acids, 22, 153-165. Assay: Hemoglobin in mouse matrigel plug.
Ninjurin 1 dodecamer peptide containing the N-terminal adhesion motif (N-NAM) exerts proangiogenic effects in HUVECs and in the postischemic brain
Kim, S., et al (2020). Ninjurin 1 dodecamer peptide containing the N-terminal adhesion motif (N-NAM) exerts proangiogenic effects in HUVECs and in the postischemic brain. Scientific Reports, 10(1). Assay: Hemoglobin in mouse matrigel plug.
Tissue distribution of 14C-labelled perfluorooctanoic acid in adult mice after 1-5 days of dietary exposure to an experimental dose or a lower dose that resulted in blood levels similar to those detected in exposed humans
Bogdanska, J., et al. (2020). Tissue distribution of 14C-labelled perfluorooctanoic acid in adult mice after 1-5 days of dietary exposure to an experimental dose or a lower dose that resulted in blood levels similar to those detected in exposed humans. Chemosphere, 239, 124755. Assay: Hemoglobin in mouse tissue.
Sequential activation of necroptosis and apoptosis cooperates to mediate vascular and neural pathology in stroke
Naito, M. G., et al (2020). Sequential activation of necroptosis and apoptosis cooperates to mediate vascular and neural pathology in stroke. Proceedings of the National Academy of Sciences, 117(9), 4959-4970. Assay: Hemoglobin in mouse blood.
Indian Journal of Novel Drug Delivery
KHAN, Z. J., KHAN, N. A., NASEEM, I., & NAMI, S. A. (2019). Indian Journal of Novel Drug Delivery. Indian Journal of Novel Drug Delivery, 11(1), 30-39. Assay: TBARS in rats blood.
Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur
Koner, D et al (2019). Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur. Nitric Oxide 84:7-15. Assay: TBARS in catfish liver cells.
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen
Pereira, B. A., Rocha, L. G. P., Teles, M. C., Silva, W. E., Barbosa, J. A., Rabelo, S. S. & Zangeronimo, M. G. (2019). Addition of chlorogenic acid and caffeine during the processing of cooled boar semen. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 71(2), 489-499. Assay: TBARS in boar plasma.
Photohydroionization Reduces Shiga Toxin-Producing Escherichia coli and Salmonella on Fresh Beef with Minimal Effects on Meat Quality
Yang, X., Kalchayanand, N., Belk, K. E., & Wheeler, T. L. (2019). Photohydroionization Reduces Shiga Toxin-Producing Escherichia coli and Salmonella on Fresh Beef with Minimal Effects on Meat Quality. Meat and Muscle Biology, 3(1), 105-115. Assay: TBARS in cow tissue.
Fatty acid composition, vitamin A content and oxidative stability of milk in China
Liang, K., Zhao, Y., Han, J., Liu, P., Qiu, J., Zhu, D. & Wang, X. (2018). Fatty acid composition, vitamin A content and oxidative stability of milk in China. Journal of Applied Animal Research, 46(1), 566-571. Assay: TBARS in cow milk.
Carcass traits, fatty acid composition, gene expression, oxidative stability and quality attributes of different muscles in Dorper lambs fed Nigella sativa seeds, Rosmarinus officinalis leaves and their combination
Odhaib, K. J., Adeyemi, K. D., & Sazili, A. Q. (2018). Carcass traits, fatty acid composition, gene expression, oxidative stability and quality attributes of different muscles in Dorper lambs fed Nigella sativa seeds, Rosmarinus officinalis leaves and their combination. Asian-Australasian journal of animal sciences, 31(8), 1345. Assay: TBARS in lamb muscle tissue.
Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows
Laubenthal, L., Ruda, L., Sultana, N., Winkler, J., Rehage, J., Meyer, U. & Haussler, S. (2017). Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows. Journal of dairy science, 100(6), 4976-4986. Assay: TBARS in cow serum.
Nrf2 modulates cell proliferation and antioxidants defenses during liver regeneration induced by partial hepatectomy
Morales-Gonzalez, A., Bautista, M., Mandrigal-Santillan, E., Posadas-Mondragon, A., Anguiano-Robledo, L., Mandrigal-Bajaidar, E. & Sanchez-Moreno, C. (2017). Nrf2 modulates cell proliferation and antioxidants defenses during liver regeneration induced by partial hepatectomy. Int. J. Clin. Exp. Pathol, 10, 7801-7811. Assay: TBARS in rats liver cells.
Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend
Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: TBARS in goats serum.
Effects of dietary oil blend on fatty acid composition, oxidative stability and physicochemical properties of Longissimus thoracis et lumborum muscle in goats
Adeyemi, KD et al (2016). Effects of dietary oil blend on fatty acid composition, oxidative stability and physicochemical properties of Longissimus thoracis et lumborum muscle in goats. Animal Science Journal 87(11):1421-1432. Assay: TBARS in goat muscle cells.
Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats
Adeyemi, KD et al (2016).Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of Animal Science and Technology. 58:23. Assay: TBARS in goat muscle cells.
Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions
Soren, S., Singh, S. V., & Singh, P. (2016). Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions. Turkish Journal of Veterinary and Animal Sciences, 40(6), 797-802. Assay: TBARS in bulls plasma.
Semen quality and reproductive performance of boars kept in pens containing conventional coffee husk as a floor covering
Teles, M. C., Pereira, B. A., Rocha, L. G. P., Resende, C. O., Rodrigues, V. V., Pereira, L. J. & Zangeronimo, M. G. (2016). Semen quality and reproductive performance of boars kept in pens containing conventional coffee husk as a floor covering. Revista Brasileira de Zootecnia, 45(7), 365-371. Assay: TBARS in pig plasma.
Amelioration of chronic cyclosporine A-induced nephrotoxicity by telmisartan in rats
Al-Amran, F.G., et al. (2011). Amelioration of chronic cyclosporine A-induced nephrotoxicity by telmisartan in rats. African Journal of Pharmacy and Pharmacology 5(4):500-505. Assay: TBARS in rat renal tissue.
Innate immune responses to a single session of sprint interval training
Davison, G. (2011). Innate immune responses to a single session of sprint interval training. Appl Physiol Nutr Metab 36(3):395-404. Assay: TBARS in human.
Lutein supplementation alters inflammatory cytokine production and antioxidant status in F-line turkeys
Shanmugasundaram, R.Selvaraj, R.K. (2011). Lutein supplementation alters inflammatory cytokine production and antioxidant status in F-line turkeys. Poult Sci 90(5):971-6. Assay: TBARS in turkey liver.
Preoperative fasting protects mice against hepatic ischemia/reperfusion injury: mechanisms and effects on liver regeneration
Verweij, M., et al. (2011). Preoperative fasting protects mice against hepatic ischemia/reperfusion injury: mechanisms and effects on liver regeneration. Liver Transpl 17(6):695-704. Assay: TBARS in mouse liver.
The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma
Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: TBARS in human blood.
Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis
Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: TBARS in Japanese quail blood.
